Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran

被引：0
作者：
Habibi, G. R. [1 ]
Imani, A. R. [2 ]
Gholami, M. R. [3 ]
Hablolvarid, M. H. [3 ]
Behroozikhah, A. M. [4 ]
Lotfi, M. [5 ]
Kamalzade, M. [5 ]
Najjar, E. [6 ]
Esmaeil-Nia, K. [1 ]
Bozorgi, S. [1 ]
机构：
[1] Razi Vaccine & Serum Res Inst, Parasite Vaccine Res & Prod Dept, Karaj, Iran
[2] Islamic Azad Univ, Vet Coll, Karaj, Iran
[3] Razi Vaccine & Serum Res Inst, Dept Pathol, Karaj, Iran
[4] Razi Vaccine & Serum Res Inst, Brucellosis Vaccine Res & Prod Dept, Karaj, Iran
[5] Razi Vaccine & Serum Res Inst, Qual Control Biol Prod Grp, Karaj, Iran
[6] Vet Org Qazvin Prov, Qazvin, Iran
来源：
IRANIAN JOURNAL OF PARASITOLOGY | 2012年 / 7卷 / 03期
关键词：
Toxoplasma gondii; Sheep abortion; Fetus; PCR; Bioassay; Genotyping; POLYMERASE-CHAIN-REACTION; CONGENITAL TOXOPLASMOSIS; PREGNANT-WOMEN; DIAGNOSIS; PCR; STRAINS; CATTLE; OVINE; HISTOPATHOLOGY; SEROPREVALENCE;
D O I：
暂无
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Background: The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran. Methods: Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopathological observation and microbiological assay. Bioassay in mice was carried out by inoculating the brain samples intraperitoneally. Results: The results demonstrated the frequency of 66% infected sheep aborted fetal samples with T. gondii type one. Although we could not isolate any parasite from inoculated mice even after three passages, but it was confirmed histopathologically formation of cyst like bodies in prepared mice brain sections. Conclusion: The results of the performed nested-PCR and formation of brain cyst in inoculated mice exhibited that T. gondii type one infection might be considered as one of the major causative agents for abortion in ewes.
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页码：64 / 72
页数：9
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