Fhl1 as a downstream target of Wnt signaling to promote myogenesis of C2C12 cells

被引:14
作者
Lee, Jing-Yu [1 ,2 ]
Chien, I-Chun [1 ]
Lin, Win-Yu [1 ]
Wu, Shao-min [1 ]
Wei, Bo-Huei [1 ]
Lee, Yu-En [1 ]
Lee, Hu-Hui [1 ]
机构
[1] Natl Chiayi Univ, Dept Bioagr Sci, Chiayi 60004, Taiwan
[2] Natl Chiayi Univ, PhD Program Agr Sci, Chiayi 60004, Taiwan
关键词
Wnt signaling; Fhl1; Myogenesis; C2C12; Cell culture; Differentiation; beta-Catenin; SKELETAL-MUSCLE; STEM-CELLS; LIM-DOMAINS; CARDIOMYOGENIC DIFFERENTIATION; SCAPULOPERONEAL MYOPATHY; ABERRANT METHYLATION; BLADDER-CANCER; GASTRIC-CANCER; BETA-CATENIN; COLON-CANCER;
D O I
10.1007/s11010-012-1266-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Previous studies have shown that Wnt signaling is involved in postnatal mammalian myogenesis; however, the downstream mechanism of Wnt signaling is not fully understood. This study reports that the murine four-and-a-half LIM domain 1 (Fhl1) could be stimulated by beta-catenin or LiCl treatment to induce myogenesis. In contrast, knockdown of the Fhl1 gene expression in C2C12 cells led to reduced myotube formation. We also adopted reporter assays to demonstrate that either beta-catenin or LiCl significantly activated the Fhl1 promoter, which contains four putative consensus TCF/LEF binding sites. Mutations of two of these sites caused a significant decrease in promoter activity by luciferase reporter assay. Thus, we suggest that Wnt signaling induces muscle cell differentiation, at least partly, through Fhl1 activation.
引用
收藏
页码:251 / 262
页数:12
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