DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis

被引：213
作者：
Liu, W [1 ]
Li, PJ [1 ]
Qi, XM [1 ]
Zhou, Q [1 ]
Zheng, L [1 ]
Sun, TH [1 ]
Yang, YS [1 ]
机构：
[1] Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
来源：
CHEMOSPHERE | 2005年 / 61卷 / 02期
基金：
中国国家自然科学基金;
关键词：
cadmium (Cd); root growth; total soluble protein content; DNA polymorphism change; genomic template stability; biomarker;
D O I：
10.1016/j.chemosphere.2005.02.078
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
In recent years, several plant species have been used as bioindicators, and several tests have been developed to evaluate the toxicity of environmental contaminants on vegetal organisms. In this study, barley (Hordeum vulgare L) seedling was used as bioindicator of cadmium (Cd) pollution in the range of 30-120 mg l(-1). Inhibition of root growth and reduction of total soluble protein content in root tips of barley seedlings were observed with the increase of Cd concentrations. The changes occurring in random amplified polymorphic DNA (RAPD) profiles of root tips following Cd treatment included variation in band intensity, loss of normal bands and appearance of new bands compared with the normal seedlings. Additionally, we found that the effect of changes was dose-dependent. These results indicated that genomic template stability (a qualitative measure reflecting changes in RAPD profiles) was significantly affected at the above Cd concentration. Thus, DNA polymorphisms detected by RAPD analysis could be used as an investigation tool for environmental toxicology and as a useful biomarker assay for the detection of genotoxic effects of Cd pollution on plants. (C) 2005 Elsevier Ltd. All rights reserved.
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收藏
页码：158 / 167
页数：10
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