Single channel recording of a mitochondrial calcium uniporter

被引：11
作者：
Wu, Guangyan [1 ,2 ]
Li, Shunjin [1 ,2 ]
Zong, Guangning [1 ,2 ,3 ]
Liu, Xiaofen [1 ,2 ]
Fei, Shuang [3 ]
Shen, Linda [4 ]
Guan, Xiangchen [1 ,2 ]
Yang, Xue [1 ,2 ]
Shen, Yuequan [1 ,2 ,5 ]
机构：
[1] Nankai Univ, State Key Lab Med Chem Biol, 94 Weijin Rd, Tianjin 300071, Peoples R China
[2] Nankai Univ, Coll Life Sci, 94 Weijin Rd, Tianjin 300071, Peoples R China
[3] Nankai Univ, Coll Pharm, 38 Tongyan Rd, Tianjin 300350, Peoples R China
[4] Tianjin Nankai High Sch, 22 Nankaisima Rd, Tianjin 300100, Peoples R China
[5] Synerget Innovat Ctr Chem Sci & Engn, 94 Weijin Rd, Tianjin 300071, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 496卷 / 01期
基金：
国家重点研发计划;
关键词：
Mitochondrial calcium uptake; Mitochondrial calcium uniporter; MCU; Calcium signaling; Single channel recording; MICU1; MCU; PROTEIN;
D O I：
10.1016/j.bbrc.2018.01.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mitochondrial calcium uniporter (MCU) is the pore-forming subunit of the entire uniporter complex and plays an important role in mitochondrial calcium uptake. However, the single channel recording of MCU remains controversial. Here, we expressed and purified different MCU proteins and then reconstituted them into planar lipid bilayers for single channel recording. We showed that MCU alone from Pyronema omphalodes (pMCU) is active with prominent single channel Ca2+ currents. In sharp contrast, MCU alone from Homo sapiens (hMCU) is inactive. The essential MCU regulator (EMRE) activates hMCU, and therefore, the complex (hMCU-hEMRE) shows prominent single channel Ca2+ currents. These single channel currents are sensitive to the specific MCU inhibitor Ruthenium Red. Our results clearly demonstrate that active MCU can conduct large amounts of calcium into the mitochondria. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：127 / 132
页数：6
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