Single channel recording of a mitochondrial calcium uniporter

被引：11
作者：
Wu, Guangyan [1 ,2 ]
Li, Shunjin [1 ,2 ]
Zong, Guangning [1 ,2 ,3 ]
Liu, Xiaofen [1 ,2 ]
Fei, Shuang [3 ]
Shen, Linda [4 ]
Guan, Xiangchen [1 ,2 ]
Yang, Xue [1 ,2 ]
Shen, Yuequan [1 ,2 ,5 ]
机构：
[1] Nankai Univ, State Key Lab Med Chem Biol, 94 Weijin Rd, Tianjin 300071, Peoples R China
[2] Nankai Univ, Coll Life Sci, 94 Weijin Rd, Tianjin 300071, Peoples R China
[3] Nankai Univ, Coll Pharm, 38 Tongyan Rd, Tianjin 300350, Peoples R China
[4] Tianjin Nankai High Sch, 22 Nankaisima Rd, Tianjin 300100, Peoples R China
[5] Synerget Innovat Ctr Chem Sci & Engn, 94 Weijin Rd, Tianjin 300071, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 496卷 / 01期
基金：
国家重点研发计划;
关键词：
Mitochondrial calcium uptake; Mitochondrial calcium uniporter; MCU; Calcium signaling; Single channel recording; MICU1; MCU; PROTEIN;
D O I：
10.1016/j.bbrc.2018.01.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mitochondrial calcium uniporter (MCU) is the pore-forming subunit of the entire uniporter complex and plays an important role in mitochondrial calcium uptake. However, the single channel recording of MCU remains controversial. Here, we expressed and purified different MCU proteins and then reconstituted them into planar lipid bilayers for single channel recording. We showed that MCU alone from Pyronema omphalodes (pMCU) is active with prominent single channel Ca2+ currents. In sharp contrast, MCU alone from Homo sapiens (hMCU) is inactive. The essential MCU regulator (EMRE) activates hMCU, and therefore, the complex (hMCU-hEMRE) shows prominent single channel Ca2+ currents. These single channel currents are sensitive to the specific MCU inhibitor Ruthenium Red. Our results clearly demonstrate that active MCU can conduct large amounts of calcium into the mitochondria. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：127 / 132
页数：6
相关论文
共 50 条
  • [31] Mitochondrial Calcium Uniporter Affects Neutrophil Bactericidal Activity during Staphylococcus aureus Infection
    Monteith, Andrew J.
    Miller, Jeanette M.
    Beavers, William N.
    Maloney, K. Nichole
    Seifert, Erin L.
    Hajnoczky, Gyorgy
    Skaar, Eric P.
    INFECTION AND IMMUNITY, 2022, 90 (02)
  • [32] The Function of the Mitochondrial Calcium Uniporter in Neurodegenerative Disorders
    Liao, Yajin
    Dong, Yuan
    Cheng, Jinbo
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (02)
  • [33] Importance of mitochondrial calcium uniporter in high glucose-induced endothelial cell dysfunction
    Chen, Wei
    Yang, Jie
    Chen, Shuhua
    Xiang, Hong
    Liu, Hengdao
    Lin, Dan
    Zhao, Shaoli
    Peng, Hui
    Chen, Pan
    Chen, Alex F.
    Lu, Hongwei
    DIABETES & VASCULAR DISEASE RESEARCH, 2017, 14 (06) : 494 - 501
  • [34] The mitochondrial calcium uniporter in the heart: energetics and beyond
    Kwong, Jennifer Q.
    JOURNAL OF PHYSIOLOGY-LONDON, 2017, 595 (12): : 3743 - 3751
  • [35] The mitochondrial calcium uniporter: Balancing tumourigenic and anti-tumourigenic responses
    Colussi, Danielle M.
    Stathopulos, Peter B.
    JOURNAL OF PHYSIOLOGY-LONDON, 2024, 602 (14): : 3315 - 3339
  • [36] The mitochondrial calcium uniporter underlies metabolic fuel preference in skeletal muscle
    Kwong, Jennifer Q.
    Huo, Jiuzhou
    Bround, Michael J.
    Boyer, Justin G.
    Schwanekamp, Jennifer A.
    Ghazal, Nasab
    Maxwell, Joshua T.
    Jang, Young C.
    Khuchua, Zaza
    Shi, Kevin
    Bers, Donald M.
    Davis, Jennifer
    Molkentin, Jeffery D.
    JCI INSIGHT, 2018, 3 (22)
  • [37] The mitochondrial calcium uniporter complex: molecular components, structure and physiopathological implications
    Marchi, Saverio
    Pinton, Paolo
    JOURNAL OF PHYSIOLOGY-LONDON, 2014, 592 (05): : 829 - 839
  • [38] Mitochondrial calcium uniporter promotes cell proliferation and migration in esophageal cancer
    Miao, Yu
    Wang, Xiaofei
    Lai, Yafang
    Lin, Wan
    Huang, Ying
    Yin, Hua
    Hou, Ruirui
    Zhang, Feixiong
    ONCOLOGY LETTERS, 2021, 22 (03)
  • [39] Diquat exposure causes brainstem demyelination by upregulating the mitochondrial calcium uniporter
    Zhou, Jie
    Yang, Yingli
    Tian, Jiacheng
    Liu, Cong
    Chen, Jiafei
    Yang, Mei
    Zhang, Mengran
    Duan, Yu
    Zhang, Tian
    Sun, Yapei
    Yu, Qin
    Xia, Zhiqin
    Wan, Xinglin
    Duan, Weixia
    Xu, Shangcheng
    JOURNAL OF HAZARDOUS MATERIALS, 2025, 492
  • [40] Mitochondrial calcium uniporter as a potential therapeutic strategy for Alzheimer's disease
    Venugopal, Anila
    Iyer, Mahalaxmi
    Balasubramanian, Venkatesh
    Vellingiri, Balachandar
    ACTA NEUROPSYCHIATRICA, 2020, 32 (02): : 65 - 71
12345