Wnt/β-catenin and Hedgehog pathways are involved in the inflammatory effect of Interleukin 18 on rat chondrocytes

被引：13

作者：

Bao, Jiapeng ^{[1
]}

Ma, Chiyuan ^{[1
]}

Ran, Jisheng ^{[1
]}

Xiong, Yan ^{[1
]}

Yan, Shigui ^{[1
]}

Wu, Lidong ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Orthoped Surg, Hangzhou 310000, Zhejiang, Peoples R China

来源：

ONCOTARGET | 2017年 / 8卷 / 66期

基金：

中国国家自然科学基金;

关键词：

Interleukin; 18; chondrocyte; osteoarthritis; Wnt/beta-catenin; Hedgehog; GAMMA-INDUCING FACTOR; MATRIX-METALLOPROTEINASE; ARTICULAR CHONDROCYTES; CARTILAGE; OSTEOARTHRITIS; ARTHRITIS; DEGRADATION; PROTEIN; FAMILY; INJURY;

D O I：

10.18632/oncotarget.20584

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To investigate the inflammatory effect of Interleukin 18 (IL-18) on rat chondrocytes and the involvement of Wnt/beta-catenin and Hedgehog pathways, the mRNA and protein level of matix-degrading enzymes (MMP-2, 3, 9,13 and aggrecanses) and chondrocyte-specific proteins (Collagen II and aggrecan) were evaluated by qRT-PCR and Western blot, and key protein level of Wnt/beta-catenin and Hedgehog pathways including beta-catenin, GSK-3 beta, DKK-1, IHH, SHH, and Gli-2 were evaluated by Western blot. Dickkopf-1 (DKK-1) and Cyclopamine were used as antagonist of Wnt/beta-catenin and Hedgehog pathways to perform pathway inhibition tests. In addition, location and expression of beta-catenin, GSK-3 beta, Gli-2 and Smo were assessed by Immunofluorescence microscopy. The results showed up-regulation of matix-degrading enzymes (MMP-2, 3, 9,13 and aggrecanses) and down-regulation of chondrocyte-specific proteins (Collagen II and aggrecan) at both mRNA and protein level and activation of Wnt/beta-catenin and Hedgehog pathways in the inflammatory reaction on rat chondrocytes caused by IL-18 treatment was observed. As conclusion, Wnt/beta-catenin and Hedgehog pathways are involved in the inflammatory effect of IL-18 in vitro.

引用

页码：109962 / 109972

页数：11

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