Wnt/β-catenin and Hedgehog pathways are involved in the inflammatory effect of Interleukin 18 on rat chondrocytes

被引：13
作者：
Bao, Jiapeng [1 ]
Ma, Chiyuan [1 ]
Ran, Jisheng [1 ]
Xiong, Yan [1 ]
Yan, Shigui [1 ]
Wu, Lidong [1 ]
机构：
[1] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Orthoped Surg, Hangzhou 310000, Zhejiang, Peoples R China
来源：
ONCOTARGET | 2017年 / 8卷 / 66期
基金：
中国国家自然科学基金;
关键词：
Interleukin; 18; chondrocyte; osteoarthritis; Wnt/beta-catenin; Hedgehog; GAMMA-INDUCING FACTOR; MATRIX-METALLOPROTEINASE; ARTICULAR CHONDROCYTES; CARTILAGE; OSTEOARTHRITIS; ARTHRITIS; DEGRADATION; PROTEIN; FAMILY; INJURY;
D O I：
10.18632/oncotarget.20584
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
To investigate the inflammatory effect of Interleukin 18 (IL-18) on rat chondrocytes and the involvement of Wnt/beta-catenin and Hedgehog pathways, the mRNA and protein level of matix-degrading enzymes (MMP-2, 3, 9,13 and aggrecanses) and chondrocyte-specific proteins (Collagen II and aggrecan) were evaluated by qRT-PCR and Western blot, and key protein level of Wnt/beta-catenin and Hedgehog pathways including beta-catenin, GSK-3 beta, DKK-1, IHH, SHH, and Gli-2 were evaluated by Western blot. Dickkopf-1 (DKK-1) and Cyclopamine were used as antagonist of Wnt/beta-catenin and Hedgehog pathways to perform pathway inhibition tests. In addition, location and expression of beta-catenin, GSK-3 beta, Gli-2 and Smo were assessed by Immunofluorescence microscopy. The results showed up-regulation of matix-degrading enzymes (MMP-2, 3, 9,13 and aggrecanses) and down-regulation of chondrocyte-specific proteins (Collagen II and aggrecan) at both mRNA and protein level and activation of Wnt/beta-catenin and Hedgehog pathways in the inflammatory reaction on rat chondrocytes caused by IL-18 treatment was observed. As conclusion, Wnt/beta-catenin and Hedgehog pathways are involved in the inflammatory effect of IL-18 in vitro.
引用
收藏
页码：109962 / 109972
页数：11
相关论文
共 36 条
  • [1] Inflammation in osteoarthritis
    Goldring, Mary B.
    Otero, Miguel
    [J]. CURRENT OPINION IN RHEUMATOLOGY, 2011, 23 (05) : 471 - 478
  • [2] Osteoarthritis: an update with relevance for clinical practice
    Bijlsma, Johannes W. J.
    Berenbaum, Francis
    Lafeber, Foris P. J. G.
    [J]. LANCET, 2011, 377 (9783) : 2115 - 2126
  • [3] Hydrostatic Pressure Regulates MicroRNA Expression Levels in Osteoarthritic Chondrocyte Cultures via the Wnt/β-Catenin Pathway
    Cheleschi, Sara
    De Palma, Anna
    Pecorelli, Alessandra
    Pascarelli, Nicola Antonio
    Valacchi, Giuseppe
    Belmonte, Giuseppe
    Carta, Serafino
    Galeazzi, Mauro
    Fioravanti, Antonella
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (01):
  • [4] Induction of advanced glycation end products and alterations of the tensile properties of articular cartilage
    Chen, AC
    Temple, MM
    Ng, DM
    Verzijl, N
    DeGroot, J
    TeKoppele, JM
    Sah, RL
    [J]. ARTHRITIS AND RHEUMATISM, 2002, 46 (12): : 3212 - 3217
  • [5] Teratogen-mediated inhibition of target tissue response to Shh signaling
    Cooper, MK
    Porter, JA
    Young, KE
    Beachy, PA
    [J]. SCIENCE, 1998, 280 (5369) : 1603 - 1607
  • [6] Implication of interleukin 18 in production of matrix metalloproteinases in articular chondrocytes in arthritis: direct effect on chondrocytes may not be pivotal
    Dai, SM
    Shan, ZZ
    Nishioka, K
    Yudoh, K
    [J]. ANNALS OF THE RHEUMATIC DISEASES, 2005, 64 (05) : 735 - 742
  • [7] Identification of the molecular response of articular cartilage to injury, by microarray screening
    Dell'Accio, Francesco
    De Bari, Cosimo
    Eltawil, Noha A.
    Vanhummelen, Paul
    Pitzalis, Costantino
    [J]. ARTHRITIS AND RHEUMATISM, 2008, 58 (05): : 1410 - 1421
  • [8] Uric acid is a danger signal of increasing risk for osteoarthritis through inflammasome activation
    Denoble, Anna E.
    Huffman, Kim M.
    Stabler, Thomas V.
    Kelly, Susan J.
    Hershfield, Michael S.
    McDaniel, Gary E.
    Coleman, R. Edward
    Kraus, Virginia B.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (05) : 2088 - 2093
  • [9] Overview of the interleukin-1 family of ligands and receptors
    Dinarello, Charles A.
    [J]. SEMINARS IN IMMUNOLOGY, 2013, 25 (06) : 389 - 393
  • [10] Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production
    Ghayur, T
    Banerjee, S
    Hugunin, M
    Butler, D
    Herzog, L
    Carter, A
    Quintal, L
    Sekut, L
    Talanian, R
    Paskind, M
    Wong, W
    Kamen, R
    Tracey, D
    Allen, H
    [J]. NATURE, 1997, 386 (6625) : 619 - 623
1234