DNA detection by dye labeled oligonucleotides using surface enhanced Raman spectroscopy

被引：9
|
作者：
Eremina, Olga E. [1 ]
Zatsepin, Timofei S. [1 ,2 ]
Farzan, Valentina M. [2 ]
Veselova, Irina A. [1 ]
Zvereva, Maria I. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia
[2] Skolkovo Innovat Ctr, Skolkovo Inst Sci & Technol, Moscow 121205, Russia
来源：
MENDELEEV COMMUNICATIONS | 2020年 / 30卷 / 01期
基金：
俄罗斯基础研究基金会;
关键词：
optical sensors; surface enhanced Raman spectroscopy; silver nanoparticles; dye labeled oligonucleotides; DNA detection; specific sequence; promoter of human telomerase catalytic subunit gene; FLUORESCENCE DETECTION; SINGLE-MOLECULE; CHARGE-TRANSFER; SILVER; SCATTERING; SERS; ACHIEVEMENTS;
D O I：
10.1016/j.mencom.2020.01.006
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Dye labeled oligonucleotides have been applied to detection of DNA with high sensitivity using surface enhanced Raman spectroscopy (SERS). Duplex formation of dye labeled oligonucleotides with DNA targets, followed by selective conjugation with silver nanoparticles, made it possible to determine the optimal distance between the SERS label and the silver surface, which was necessary for strong signal enhancement. For all Rh6G labeled oligonucleotides the limits of detection were 3 fmol dm(-3), while for Cy3 labeled oligonucleotides the signal enhancement depended on the distance between Cy3 dye and silver nanoparticles.
引用
收藏
页码：18 / 21
页数：4
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