DNA detection by dye labeled oligonucleotides using surface enhanced Raman spectroscopy

被引：9

作者：

Eremina, Olga E. ^{[1
]}

Zatsepin, Timofei S. ^{[1
,2
]}

Farzan, Valentina M. ^{[2
]}

Veselova, Irina A. ^{[1
]}

Zvereva, Maria I. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia

[2] Skolkovo Innovat Ctr, Skolkovo Inst Sci & Technol, Moscow 121205, Russia

来源：

MENDELEEV COMMUNICATIONS | 2020年 / 30卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

optical sensors; surface enhanced Raman spectroscopy; silver nanoparticles; dye labeled oligonucleotides; DNA detection; specific sequence; promoter of human telomerase catalytic subunit gene; FLUORESCENCE DETECTION; SINGLE-MOLECULE; CHARGE-TRANSFER; SILVER; SCATTERING; SERS; ACHIEVEMENTS;

D O I：

10.1016/j.mencom.2020.01.006

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Dye labeled oligonucleotides have been applied to detection of DNA with high sensitivity using surface enhanced Raman spectroscopy (SERS). Duplex formation of dye labeled oligonucleotides with DNA targets, followed by selective conjugation with silver nanoparticles, made it possible to determine the optimal distance between the SERS label and the silver surface, which was necessary for strong signal enhancement. For all Rh6G labeled oligonucleotides the limits of detection were 3 fmol dm(-3), while for Cy3 labeled oligonucleotides the signal enhancement depended on the distance between Cy3 dye and silver nanoparticles.

引用

页码：18 / 21

页数：4

共 29 条

[1] Tuning SERS for living erythrocytes: Focus on nanoparticle size and plasmon resonance position
Brazhe, N. A.
Parshina, E. Y.
Khabatova, V. V.
Semenova, A. A.
Brazhe, A. R.
Yusipovich, A. I.
Sarycheva, A. S.
Churin, A. A.
Goodilin, E. A.
Maksimov, G. V.
Sosnovtseva, O. V.
[J]. JOURNAL OF RAMAN SPECTROSCOPY, 2013, 44 (05) : 686 - 694
[2] Surface-enhanced Raman spectroscopy in modern chemical analysis: advances and prospects
Eremina, Olga E.
Semenova, Anna A.
Sergeeva, Elena A.
Brazhe, Nadezhda A.
Maksimov, Georgy V.
Shekhovtsova, Tatyana N.
Goodilin, Evgene A.
Veselova, Irina A.
[J]. RUSSIAN CHEMICAL REVIEWS, 2018, 87 (08) : 741 - 770
[3] Novel Multilayer Nanostructured Materials for Recognition of Polycyclic Aromatic Sulfur Pollutants and Express Analysis of Fuel Quality and Environmental Health by Surface Enhanced Raman Spectroscopy
Eremina, Olga E.
Sidorov, Alexander V.
Shekhovtsova, Tatyana N.
Goodilin, Eugene A.
Veselova, Irina A.
[J]. ACS APPLIED MATERIALS & INTERFACES, 2017, 9 (17) : 15058 - 15067
[4] Automated Solid-Phase Click Synthesis of Oligonucleotide Conjugates: From Small Molecules to Diverse N-Acetylgalactosamine Clusters
Farzan, Valentina M.
Ulashchik, Egor A.
Martynenko-Makaev, Yury V.
Kvach, Maksim V.
Aparin, Ilya O.
Brylev, Vladimir A.
Prikazchikova, Tatiana A.
Maldakova, Svetlana Yu.
Majouga, Alexander G.
Ustinov, Alexey V.
Shipulin, German A.
Shmanai, Vadim V.
Korshun, Vladimir A.
Zatsepin, Timofei S.
[J]. BIOCONJUGATE CHEMISTRY, 2017, 28 (10) : 2599 - 2607
[5] SERRS as a more sensitive technique for the detection of labelled oligonucleotides compared to fluorescence
Faulds, K
Barbagallo, RP
Keer, JT
Smith, WE
Graham, D
[J]. ANALYST, 2004, 129 (07) : 567 - 568
[6] Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Faulds, K
Smith, WE
Graham, D
[J]. ANALYTICAL CHEMISTRY, 2004, 76 (02) : 412 - 417
[7] Flusberg BA, 2010, NAT METHODS, V7, P461, DOI [10.1038/nmeth.1459, 10.1038/NMETH.1459]
[8] Control of enhanced Raman scattering using a DNA-based assembly process of dye-coded nanoparticles
Graham, Duncan
Thompson, David G.
Smith, W. Ewen
Faulds, Karen
[J]. NATURE NANOTECHNOLOGY, 2008, 3 (09) : 548 - 551
[9] Achievements and Peculiarities in Studies of Ancient DNA and DNA from Complicated Forensic Specimens
Grigorenko, A. P.
Borinskaya, S. A.
Yankovsky, N. K.
Rogaev, E. I.
[J]. ACTA NATURAE, 2009, 1 (03): : 58 - 69
[10] DNA-Protected Silver Clusters for Nanophotonics
Gwinn, Elisabeth
Schultz, Danielle
Copp, Stacy M.
Swasey, Steven
[J]. NANOMATERIALS, 2015, 5 (01): : 180 - 207

← 1 2 3 →