DNA detection by dye labeled oligonucleotides using surface enhanced Raman spectroscopy

被引：9
|
作者：
Eremina, Olga E. [1 ]
Zatsepin, Timofei S. [1 ,2 ]
Farzan, Valentina M. [2 ]
Veselova, Irina A. [1 ]
Zvereva, Maria I. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia
[2] Skolkovo Innovat Ctr, Skolkovo Inst Sci & Technol, Moscow 121205, Russia
来源：
MENDELEEV COMMUNICATIONS | 2020年 / 30卷 / 01期
基金：
俄罗斯基础研究基金会;
关键词：
optical sensors; surface enhanced Raman spectroscopy; silver nanoparticles; dye labeled oligonucleotides; DNA detection; specific sequence; promoter of human telomerase catalytic subunit gene; FLUORESCENCE DETECTION; SINGLE-MOLECULE; CHARGE-TRANSFER; SILVER; SCATTERING; SERS; ACHIEVEMENTS;
D O I：
10.1016/j.mencom.2020.01.006
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Dye labeled oligonucleotides have been applied to detection of DNA with high sensitivity using surface enhanced Raman spectroscopy (SERS). Duplex formation of dye labeled oligonucleotides with DNA targets, followed by selective conjugation with silver nanoparticles, made it possible to determine the optimal distance between the SERS label and the silver surface, which was necessary for strong signal enhancement. For all Rh6G labeled oligonucleotides the limits of detection were 3 fmol dm(-3), while for Cy3 labeled oligonucleotides the signal enhancement depended on the distance between Cy3 dye and silver nanoparticles.
引用
收藏
页码：18 / 21
页数：4
相关论文
共 50 条
  • [1] Development of a dye-labeled DNA system for detection of TERT promoter region by surface enhanced Raman spectroscopy
    Pisarev, E.
    Kapitanova, O.
    Zatsepin, T.
    Veselova, I.
    Ogurtsova, A.
    Eremina, O.
    Zvereva, M.
    FEBS OPEN BIO, 2021, 11 : 250 - 250
  • [2] Detection and identification of labeled DNA by surface enhanced resonance Raman scattering
    Graham, D
    Mallinder, BJ
    Smith, WE
    BIOPOLYMERS, 2000, 57 (02) : 85 - 91
  • [3] Detection of integrins using surface enhanced Raman Spectroscopy
    Gant, VA
    Chowdury, MH
    Meininger, GA
    Coté, GL
    OPTICAL DIAGNOSTICS AND SENSING IV, 2004, 5325 : 174 - 178
  • [4] Label-Free Detection of DNA Hybridization Using Surface Enhanced Raman Spectroscopy
    Barhoumi, Aoune
    Halas, Naomi J.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (37) : 12792 - 12793
  • [5] Detection of DNA hybridization using electrochemical impedance spectroscopy and surface enhanced Raman scattering
    Gruetzke, Stefanie
    Abdali, Salim
    Schuhmann, Wolfgang
    Gebala, Magdalena
    ELECTROCHEMISTRY COMMUNICATIONS, 2012, 19 : 59 - 62
  • [6] Surface-plasmon-enhanced fluorescence spectroscopy for DNA detection using fluorescently labeled PNA as "DNA indicator"
    Chu, Li-Qiang
    Foerch, Renate
    Knoll, Wolfgang
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (26) : 4944 - 4947
  • [7] Surface enhanced Raman spectroscopy for the detection of pathogenic DNA and protein in foods
    Chowdhury, MH
    Atkinson, B
    Good, TA
    Coté, GL
    OPTICAL DIAGNOSTICS AND SENSING IN BIOMEDICINE III, 2003, 4965 : 111 - 115
  • [8] Surface-enhanced Raman spectroscopy of DNA
    Barhoumi, Aoune
    Zhang, Dongmao
    Tam, Felicia
    Halas, Naomi J.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (16) : 5523 - 5529
  • [9] Design of a silver nanoparticle for sensitive surface enhanced Raman spectroscopy detection of carmine dye
    Wu, Yan-xiong
    Liang, Pei
    Dong, Qian-min
    Bai, Yang
    Yu, Zhi
    Huang, Jie
    Zhong, Yuan
    Dai, Yu-Chan
    Ni, Dejiang
    Shu, Hai-bo
    Pittman, Charles U., Jr.
    FOOD CHEMISTRY, 2017, 237 : 974 - 980
  • [10] Plasma-Treated Graphene Surfaces for Trace Dye Detection Using Surface-Enhanced Raman Spectroscopy
    Singh, Ningthoujam Somorjit
    Mayanglambam, Franco
    Nemade, Harshal B.
    Giri, P. K.
    ACS APPLIED NANO MATERIALS, 2022, 5 (05) : 6352 - 6364
12345