Membrane-protein crystals for neutron diffraction

被引：8

作者：

Sorensen, Thomas Lykke-Moller ^{[1
]}

Hjorth-Jensen, Samuel John ^{[1
]}

Oksanen, Esko ^{[2
,3
]}

Andersen, Jacob Lauwring ^{[4
]}

Olesen, Claus ^{[4
]}

Moller, Jesper Vuust ^{[4
]}

Nissen, Poul ^{[1
]}

机构：

[1] Aarhus Univ, Dept Mol Biol & Genet, DANDRITE, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark

[2] European Spallat Source ERIC, POB 176, S-22100 Lund, Sweden

[3] Lund Univ, Dept Biochem & Struct Biol, POB 124, S-22100 Lund, Sweden

[4] Aarhus Univ, Dept Biomed, Ole Worn Alle 3, DK-8000 Aarhus C, Denmark

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2018年 / 74卷

基金：

欧盟地平线“2020”;

关键词：

neutron macromolecular crystallography; membrane-protein crystallization; structural biology; SERCA1; CALCIUM-PUMP; X-RAY; CYCLOPIAZONIC ACID; STRUCTURAL-CHANGES; CRYSTALLOGRAPHY; ION; CRYSTALLIZATION; TRANSPORT; CA2+-ATPASE; BINDING;

D O I：

10.1107/S2059798318012561

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Neutron macromolecular crystallography (NMX) has the potential to provide the experimental input to address unresolved aspects of transport mechanisms and protonation in membrane proteins. However, despite this clear scientific motivation, the practical challenges of obtaining crystals that are large enough to make NMX feasible have so far been prohibitive. Here, the potential impact on feasibility of a more powerful neutron source is reviewed and a strategy for obtaining larger crystals is formulated, exemplified by the calcium-transporting ATPase SERCA1. The challenges encountered at the various steps in the process from crystal nucleation and growth to crystal mounting are explored, and it is demonstrated that NMX-compatible membrane-protein crystals can indeed be obtained.

引用

收藏

页码：1208 / 1218

页数：11

相关论文

共 67 条

[11] Identification and characterization of a bacterial hydrosulphide ion channel [J].

Czyzewski, Bryan K. ;

Wang, Da-Neng .

NATURE, 2012, 483 (7390) :494-U155

[12] Features and development of Coot [J].

Emsley, P. ;

Lohkamp, B. ;

Scott, W. G. ;

Cowtan, K. .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2010, 66 :486-501

[13] The Ca2+-ATPase pump facilitates bidirectional proton transport across the sarco/endoplasmic reticulum [J].

Espinoza-Fonseca, L. Michel .

MOLECULAR BIOSYSTEMS, 2017, 13 (04) :633-637

[14] Crystal Structure of a Yeast Aquaporin at 1.15 Å Reveals a Novel Gating Mechanism [J].

Fischer, Gerhard ;

Kosinska-Eriksson, Urszula ;

Aponte-Santamaria, Camilo ;

Palmgren, Madelene ;

Geijer, Cecilia ;

Hedfalk, Kristina ;

Hohmann, Stefan ;

de Groot, Bert L. ;

Neutze, Richard ;

Lindkvist-Petersson, Karin .

PLOS BIOLOGY, 2009, 7 (06)

[15] One Crystal, Two Temperatures: Cryocooling Penalties Alter Ligand Binding to Transient Protein Sites [J].

Fischer, Marcus ;

Shoichet, Brian K. ;

Fraser, James S. .

CHEMBIOCHEM, 2015, 16 (11) :1560-1564

[16] Current trends in protein crystallization [J].

Gavira, Jose A. .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2016, 602 :3-11

[17] Room Temperature Neutron Crystallography of Drug Resistant HIV-1 Protease Uncovers Limitations of X-ray Structural Analysis at 100 K [J].

Gerlits, Oksana ;

Keen, David A. ;

Blakeley, Matthew P. ;

Louis, John M. ;

Weber, Irene T. ;

Kovalevskyk, Andrey .

JOURNAL OF MEDICINAL CHEMISTRY, 2017, 60 (05) :2018-2025

[18] HiLiDe-Systematic Approach to Membrane Protein Crystallization in Lipid and Detergent [J].

Gourdon, Pontus ;

Andersen, Jacob Lauwring ;

Hein, Kim Langmach ;

Bublitz, Maike ;

Pedersen, Bjorn Panyella ;

Liu, Xiang-Yu ;

Yatime, Laure ;

Nyblom, Maria ;

Nielsen, Thorbjorn Terndrup ;

Olesen, Claus ;

Moller, Jesper Vuust ;

Nissen, Poul ;

Morth, Jens Preben .

CRYSTAL GROWTH & DESIGN, 2011, 11 (06) :2098-2106

[19] Where is crystallography going? [J].

Grimes, Jonathan M. ;

Hall, David R. ;

Ashton, Alun W. ;

Evans, Gwyndaf ;

Owen, Robin L. ;

Wagner, Armin ;

McAuley, Katherine E. ;

von Delft, Frank ;

Orville, Allen M. ;

Sorensen, Thomas ;

Walsh, Martin A. ;

Ginn, Helen M. ;

Stuart, David I. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2018, 74 :152-166

[20] Structure and activity of tryptophan-rich TSPO proteins [J].

Guo, Youzhong ;

Kalathur, Ravi C. ;

Liu, Qun ;

Kloss, Brian ;

Bruni, Renato ;

Ginter, Christopher ;

Kloppmann, Edda ;

Rost, Burkhard ;

Hendrickson, Wayne A. .

SCIENCE, 2015, 347 (6221) :551-555

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