Method for monitoring of the protein amino group changes during carbonylation

被引：21

作者：

Acimovic, Jelena M. ^{[1
]}

Jovanovic, Vesna B. ^{[1
]}

Veselinovic, Milica R. ^{[1
]}

Serckovic, Vesna Dimitrijevic ^{[2
]}

Mandic, Ljuba M. ^{[1
]}

机构：

[1] Univ Belgrade, Dept Biochem, Fac Chem, Belgrade 11158, Serbia

[2] Clin Ctr Serbia, Inst Endocrinol Diabet & Metab Dis, Belgrade, Serbia

来源：

CLINICAL BIOCHEMISTRY | 2011年 / 44卷 / 12期

关键词：

Protein amino group; Carbonylation; Methylglyoxal; Diabetes; Spectrophotometric determination with p-benzoquinone; ADVANCED GLYCATION ENDPRODUCT; SERUM-ALBUMIN; MAILLARD REACTION; GLYCEMIC CONTROL; METHYLGLYOXAL; PLASMA; PRODUCTS; ACIDS; BENZOQUINONE; HBA(1C);

D O I：

10.1016/j.clinbiochem.2011.05.019

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: Carbonylation of the protein amino, guanidino and thiol groups inane of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation. Design and methods: The method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation in vitro with methylglyoxal and in vivo in 13 patients with type 2 diabetes and 20 healthy persons. Results: The method is simple, fast, precise (RSD in the range of 1.2-1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p<0.01) in comparison with a control group. Conclusion: The method developed is suitable for quantification of protein amino groups during in vitro carbonylation as well as for clinical practice. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：994 / 999

页数：6

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