Multi-material digital light processing bioprinting of hydrogel-based microfluidic chips

被引：72

作者：

Bhusal, Anant ^{[1
]}

Dogan, Elvan ^{[1
]}

Nguyen, Hai-Anh ^{[1
]}

Labutina, Olga ^{[1
]}

Nieto, Daniel ^{[2
]}

Khademhosseini, Ali ^{[3
,4
,5
,6
]}

Miri, Amir K. ^{[1
,7
]}

机构：

[1] Rowan Univ, Dept Mech Engn, Glassboro, NJ 08028 USA

[2] Univ Santiago de Compostela, Dept Phys, Photon 4life Res Grp, La Coruna, Spain

[3] Terasaki Inst Biomed Innovat TIBI, Los Angeles, CA 90024 USA

[4] Univ Calif Los Angeles, Dept Radiol, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA

[6] Univ Calif Los Angeles, Henry Samueli Sch Engn & Appl Sci, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA

[7] Rowan Univ, Dept Biomed Engn, Glassboro, NJ 08028 USA

来源：

BIOFABRICATION | 2022年 / 14卷 / 01期

基金：

美国国家卫生研究院;

关键词：

digital-light-processing; organ-on-a-chip; microfluidics; hydrogel models; BIOMEDICAL APPLICATIONS; CELL-CULTURE; GELATIN; ORGANS; TECHNOLOGIES; FABRICATION; STIFFNESS; MODEL;

D O I：

10.1088/1758-5090/ac2d78

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Recent advancements in digital-light-processing (DLP)-based bioprinting and hydrogel engineering have enabled novel developments in organs-on-chips. In this work, we designed and developed a multi-material, DLP-based bioprinter for rapid, one-step prototyping of hydrogel-based microfluidic chips. A composite hydrogel bioink based on poly-ethylene-glycol-diacrylate (PEGDA) and gelatin methacryloyl (GelMA) was optimized through varying the bioprinting parameters such as light exposure time, bioink composition, and layer thickness. We showed a wide range of mechanical properties of the microfluidic chips for various ratios of PEGDA:GelMA. Microfluidic features of hydrogel-based chips were then tested using dynamic flow experiments. Human-derived tumor cells were encapsulated in 3D bioprinted structures to demonstrate their bioactivity and cell-friendly environment. Cell seeding experiments then validated the efficacy of the selected bioinks for vascularized micro-tissues. Our biofabrication approach offers a useful tool for the rapid integration of micro-tissue models into organs-on-chips and high-throughput drug screening platforms.

引用

页数：13

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