UV irradiation upregulates Egr-1 expression at transcription level

被引：0
作者：
Huang, RP [1 ]
Fan, Y [1 ]
Boynton, AL [1 ]
机构：
[1] NW Hosp, Seattle, WA 98125 USA
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 1999年 / 73卷 / 02期
关键词：
Egr-1; gene expression; transcription; uv irradiation;
D O I：
10.1002/(SICI)1097-4644(19990501)73:2<227::AID-JCB9>3.0.CO;2-B
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
UVC irradiation rapidly and strongly induces protein expression of the early growth response-1 gene (Egr-1) encoding a transcription factor which may have a protective function against UV damage. In this paper, we further investigate mechanisms responsible for such induction. We show that UVC irradiation also induced Egr-1 mRNA expression, increased transcription rate by nuclear run-on assay and stimulated Egr-1 promoter activity by CAT assay. The Egr-1 mRNA stability remained unchanged in UVC-treated cells. On the other hand, UVC irradiation slightly extended Egr-1 protein half-life. The induction of Egr-1 by UVC was observed in many different cell types. UVA and UVB also strongly induced Egr-1 expression. These results indicate that UVC regulates Egr-1 expression at transcription level. The induction pattern of Egr-1 by UV suggests the importance of Egr-1 in the UV response.. (C) 1999 Wiley-Liss, Inc.
引用
收藏
页码：227 / 236
页数：10
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