A comparison of gel filtration chromatographic supports for plasmid purification

被引：45

作者：

Ferreira, GNM ^{[1
]}

Cabral, JMS ^{[1
]}

Prazeres, DMF ^{[1
]}

机构：

[1] Univ Tecn Lisboa, CTR ENGN BIOL & QUIM, INST SUPER TECN, ENGN BIOQUIM LAB, P-1096 LISBON, PORTUGAL

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 06期

关键词：

D O I：

10.1023/A:1018420807203

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two gel filtration chromatographic supports, Superose 6 and Sephacryl S1000 SF, were used to purify supercoiled plasmids using a 4.8 kb plasmid as a model. Both supports purified the plasmid from RNA and other small molecular weight contaminants, as shown by agarose electrophoresis and ion exchange HPLC, with an overall yield of 70%. Sephacryl S1000 SF was the better support as it resolved supercoiled, relaxed, linear and concatamer plasmid forms, and chromosomal DNA.

引用

页码：417 / 420

页数：4