Self calibration of sensorless adaptive optical microscopes

被引:18
作者
Thayil, Anisha [1 ]
Booth, Martin J. [1 ]
机构
[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
来源
JOURNAL OF THE EUROPEAN OPTICAL SOCIETY-RAPID PUBLICATIONS | 2011年 / 6卷
基金
英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;
关键词
Adaptive optics; aberrations; microscopy; MEMBRANE MIRROR;
D O I
10.2971/jeos.2011.11045
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We present a self-calibrating scheme for microscopes using model-based wavefront sensorless adaptive optics. Unlike previous methods, this scheme permits the calibration of system aberration modes without the need for a separate wavefront sensor or interferometer. Basis modes are derived from the deformable mirror influence functions and an image cross-correlation method is used to remove image displacement effects from these modes. Image based measurements are used to derive an optimum modal representation from the displacement-free basis modes. These new modes are insensitive to system misalignments and the shape of the illumination profile. We demonstrate the effectiveness and robustness of these optimal modes in a third harmonic generation (THG) microscope. [DOI: http: //dx.doi.org/10.2971/jeos.2011.11045]
引用
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页数:8
相关论文
共 11 条
[1]   Adaptive optics in microscopy [J].
Booth, Martin J. .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 2007, 365 (1861) :2829-2843
[2]   Wavefront sensorless adaptive optics for large aberrations [J].
Booth, Martin J. .
OPTICS LETTERS, 2007, 32 (01) :5-7
[3]   Wave front sensor-less adaptive optics: a model-based approach using sphere packings [J].
Booth, MJ .
OPTICS EXPRESS, 2006, 14 (04) :1339-1352
[4]   Adaptive optics for structured illumination microscopy [J].
Debarre, Delphine ;
Botcherby, Edward J. ;
Booth, Martin J. ;
Wilson, Tony .
OPTICS EXPRESS, 2008, 16 (13) :9290-9305
[5]   Image based adaptive optics through optimisation of low spatial frequencies [J].
Debarre, Delphine ;
Booth, Martin J. ;
Wilson, Tony .
OPTICS EXPRESS, 2007, 15 (13) :8176-8190
[6]   Image-based adaptive optics for two-photon microscopy [J].
Debarre, Delphine ;
Botcherby, Edward J. ;
Watanabe, Tomoko ;
Srinivas, Shankar ;
Booth, Martin J. ;
Wilson, Tony .
OPTICS LETTERS, 2009, 34 (16) :2495-2497
[7]   MEMBRANE MIRROR AS AN ADAPTIVE OPTICAL ELEMENT [J].
GROSSO, RP ;
YELLIN, M .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA, 1977, 67 (03) :399-406
[8]   Adaptive harmonic generation microscopy of mammalian embryos [J].
Jesacher, Alexander ;
Thayil, Anisha ;
Grieve, Kate ;
Debarre, Delphine ;
Watanabe, Tomoko ;
Wilson, Tony ;
Srinivas, Shankar ;
Booth, Martin .
OPTICS LETTERS, 2009, 34 (20) :3154-3156
[9]   Dynamic aberration correction for multiharmonic microscopy [J].
Olivier, Nicolas ;
Debarre, Delphine ;
Beaurepaire, Emmanuel .
OPTICS LETTERS, 2009, 34 (20) :3145-3147
[10]   A low cost adaptive optics system using a membrane mirror [J].
Paterson, C ;
Munro, I ;
Dainty, JC .
OPTICS EXPRESS, 2000, 6 (09) :175-185