Phenotypic and Phylogenetic Analysis of Lactic Acid Bacteria Isolated from Forage Crops and Grasses in the Tibetan Plateau

被引：47

作者：

Pang, Huili ^{[1
]}

Tan, Zhongfang ^{[1
]}

Qin, Guangyong ^{[1
]}

Wang, Yanping ^{[1
]}

Li, Zongwei ^{[1
]}

Jin, Qingsheng ^{[2
]}

Cai, Yimin ^{[3
]}

机构：

[1] Zhengzhou Univ, Henan Prov Key Lab Ion Beam Bioengn, Zhengzhou 450052, Henan, Peoples R China

[2] Zhejiang Acad Agr Sci, Inst Crops & Utilizat Nucl Technol, Hangzhou 310021, Zhejiang, Peoples R China

[3] Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3058686, Japan

来源：

JOURNAL OF MICROBIOLOGY | 2012年 / 50卷 / 01期

基金：

中国国家自然科学基金;

关键词：

forage; epiphytic microflora; lactic acid bacteria; 16S rDNA; recA gene; SEQUENCE-ANALYSIS; SILAGE; IDENTIFICATION; PARAPLANTARUM; MODEL;

D O I：

10.1007/s12275-012-1284-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A total of 140 lactic acid bacteria (LAB) strains were isolated from corn, alfalfa, clover, sainfoin, and Indian goosegrass in the Tibetan Plateau. According to phenotypic and chemo-taxonomic characteristics, 16S rDNA sequence, and recA gene PCR amplification, these LAB isolates were identified as belonging to five genera and nine species. Corn contained more LAB species than other forage crops. Leuconostoc pseudomesenteroides, Lactococcus lactis subsp. lactis, Lactobacillus brevis, and Weissella paramesenteroides were dominant members of the LAB population on alfalfa, clover, sainfoin, and Indian goosegrass, respectively. The comprehensive 16S rDNA and recA-based approach effectively described the LAB community structure of the relatively abundant LAB species distributed on different forage crops. This is the first report describing the diversity and natural populations of LAB associated with Tibetan forage crops, and most isolates grow well at or below 10 degrees C. The results will be valuable for the future design of appropriate inoculants for silage fermentation in this very cold area.

引用

页码：63 / 71

页数：9

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