Phenotypic and Phylogenetic Analysis of Lactic Acid Bacteria Isolated from Forage Crops and Grasses in the Tibetan Plateau

被引：47

作者：

Pang, Huili ^{[1
]}

Tan, Zhongfang ^{[1
]}

Qin, Guangyong ^{[1
]}

Wang, Yanping ^{[1
]}

Li, Zongwei ^{[1
]}

Jin, Qingsheng ^{[2
]}

Cai, Yimin ^{[3
]}

机构：

[1] Zhengzhou Univ, Henan Prov Key Lab Ion Beam Bioengn, Zhengzhou 450052, Henan, Peoples R China

[2] Zhejiang Acad Agr Sci, Inst Crops & Utilizat Nucl Technol, Hangzhou 310021, Zhejiang, Peoples R China

[3] Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3058686, Japan

来源：

JOURNAL OF MICROBIOLOGY | 2012年 / 50卷 / 01期

基金：

中国国家自然科学基金;

关键词：

forage; epiphytic microflora; lactic acid bacteria; 16S rDNA; recA gene; SEQUENCE-ANALYSIS; SILAGE; IDENTIFICATION; PARAPLANTARUM; MODEL;

D O I：

10.1007/s12275-012-1284-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A total of 140 lactic acid bacteria (LAB) strains were isolated from corn, alfalfa, clover, sainfoin, and Indian goosegrass in the Tibetan Plateau. According to phenotypic and chemo-taxonomic characteristics, 16S rDNA sequence, and recA gene PCR amplification, these LAB isolates were identified as belonging to five genera and nine species. Corn contained more LAB species than other forage crops. Leuconostoc pseudomesenteroides, Lactococcus lactis subsp. lactis, Lactobacillus brevis, and Weissella paramesenteroides were dominant members of the LAB population on alfalfa, clover, sainfoin, and Indian goosegrass, respectively. The comprehensive 16S rDNA and recA-based approach effectively described the LAB community structure of the relatively abundant LAB species distributed on different forage crops. This is the first report describing the diversity and natural populations of LAB associated with Tibetan forage crops, and most isolates grow well at or below 10 degrees C. The results will be valuable for the future design of appropriate inoculants for silage fermentation in this very cold area.

引用

页码：63 / 71

页数：9

共 22 条

[1] Advantage of using inosine at the 3′ termini of 16S rRNA gene universal primers for the study of microbial diversity [J].

Ben-Dov, Eitan ;

Shapiro, Orr H. ;

Siboni, Nachshon ;

Kushmaro, Ariel .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (11) :6902-6906

[2]

Cai YM, 1998, APPL ENVIRON MICROB, V64, P2982

[3] Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage [J].

Cai, YM ;

Benno, Y ;

Ogawa, M ;

Kumai, S .

JOURNAL OF DAIRY SCIENCE, 1999, 82 (03) :520-526

[4] Identification and characterization of Enterococcus species isolated from forage crops and their influence on silage fermentation [J].

Cai, YM .

JOURNAL OF DAIRY SCIENCE, 1999, 82 (11) :2466-2471

[5]

Cai YM, 1999, APPL ENVIRON MICROB, V65, P2901

[6] Lactobacillus paraplantarum sp. nov., a new species related to Lactobacillus plantarum [J].

Curk, MC ;

Hubert, JC ;

Bringel, F .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1996, 46 (02) :595-598

[7] Influence of the Tibetan Plateau on the summer climate patterns over Asia in the IAP/LASG SAMIL model [J].

Duan Anmin ;

Wu Guoxiong ;

Liang Xiaoyun .

ADVANCES IN ATMOSPHERIC SCIENCES, 2008, 25 (04) :518-528

[8]

Eisen JA, 1995, J MOL EVOL, V41, P1105

[9] Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis [J].

Ennahar, S ;

Cai, YM ;

Fujita, Y .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (01) :444-451

[10] A STUDY OF MASTITIS BACTERIA AND HERD MANAGEMENT-PRACTICES TO IDENTIFY THEIR RELATIONSHIP TO HIGH SOMATIC-CELL COUNTS IN BULK TANK MILK [J].

FENLON, DR ;

LOGUE, DN ;

GUNN, J ;

WILSON, J .

BRITISH VETERINARY JOURNAL, 1995, 151 (01) :17-25

← 1 2 3 →