The MutS C terminus is essential for mismatch repair activity in vivo

被引：24
作者：
Calmann, MA [1 ]
Nowosielska, A [1 ]
Marinus, MG [1 ]
机构：
[1] Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA 01605 USA
来源：
JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 18期
关键词：
D O I：
10.1128/JB.187.18.6577-6579.2005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An Escherichia coli K-12 strain was constructed with a chromosomal deletion (mutS Delta 800) in the mutS gene that produced the removal of the C-terminal 53 amino acids which are not present in the MutS crystal structure. This strain has a MutS null phenotype for mutation avoidance, antirecombination, and sensitivity to cytotoxic agents in a dam mutant background.
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页码：6577 / 6579
页数：3
相关论文
共 16 条
  • [1] Oligomerization of a MutS mismatch repair protein from Thermus aquaticus
    Biswas, I
    Ban, C
    Fleming, KG
    Qin, J
    Lary, JW
    Yphantis, DA
    Yang, W
    Hsieh, P
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (33) : 23673 - 23678
  • [2] Disruption of the helix-u-turn-helix motif of MutS protein: Loss of subunit dimerization, mismatch binding and ATP hydrolysis
    Biswas, I
    Obmolova, G
    Takahashi, M
    Herr, A
    Newman, MA
    Yang, W
    Hsieh, P
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2001, 305 (04) : 805 - 816
  • [3] Assembly and molecular activities of the MutS tetramer
    Bjornson, KP
    Blackwell, LJ
    Sage, H
    Baitinger, C
    Allen, D
    Modrich, P
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (36) : 34667 - 34673
  • [4] Separation of mutation avoidance and antirecombination functions in an Escherichia coli mutS mutant
    Calmann, MA
    Nowosielska, A
    Marinus, MG
    [J]. NUCLEIC ACIDS RESEARCH, 2005, 33 (04) : 1193 - 1200
  • [5] Depletion of the cellular amounts of the MutS and MutH methyl-directed mismatch repair proteins in stationary-phase Escherichia coli K-12 cells
    Feng, G
    Tsui, HCT
    Winkler, ME
    [J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (08) : 2388 - 2396
  • [6] FRAM RJ, 1985, MOL PHARMACOL, V28, P51
  • [7] KCNE3 truncation mutants reveal a bipartite modulation of KCNQ1 K+ channels
    Gage, SD
    Kobertz, WR
    [J]. JOURNAL OF GENERAL PHYSIOLOGY, 2004, 124 (06) : 759 - 771
  • [8] JONES M, 1981, MOL GEN GENET, V184, P562, DOI 10.1007/BF00352542
  • [9] MISMATCH CORRECTION AT O-6-METHYLGUANINE RESIDUES IN ESCHERICHIA-COLI DNA
    KARRAN, P
    MARINUS, MG
    [J]. NATURE, 1982, 296 (5860) : 868 - 869
  • [10] The crystal structure of DNA mismatch repair protein MutS binding to a G•T mismatch
    Lamers, MH
    Perrakis, A
    Enzlin, JH
    Winterwerp, HHK
    de Wind, N
    Sixma, TK
    [J]. NATURE, 2000, 407 (6805) : 711 - 717
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