Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: Role of reactive nitrogen species and cyclooxygenase enzyme inhibition

被引：78

作者：

Abdelwahab, Siddig Ibrahim ^{[1
]}

Hassan, Loiy Elsir Ahmed ^{[2
]}

Sirat, Hasnah Mohd

Yagi, Sakina M. Ahmed ^{[2
]}

Koko, Waleed S. ^{[3
]}

Mohan, Syam ^{[4
]}

Taha, Manal Mohamed Elhassan ^{[4
]}

Ahmad, Syahida ^{[5
]}

Chuen, Cheah Shiau ^{[4
]}

Narrima, Putri ^{[4
]}

Rais, Mohd Mustafa ^{[4
]}

Hadi, A. Hamid A. ^{[4
]}

机构：

[1] Univ Malaya, Dept Pharm, Fac Med, Kuala Lumpur 50603, Malaysia

[2] Univ Khartoum, Dept Bot, Fac Sci, Khartoum, Sudan

[3] Med & Aromat Plant Res Inst, Khartoum, Sudan

[4] Univ Malaya, Ctr Nat Prod & Drug Discovery CENAR, Dept Pharmacol, Fac Med, Kuala Lumpur 50603, Malaysia

[5] Univ Putra Malaysia, Fac Biotechnol, Serdang, Malaysia

来源：

FITOTERAPIA | 2011年 / 82卷 / 08期

关键词：

Cucurbitacin E; Wild melon; Cyclooxygenase enzymes: reactive nitrogen species; RAW; 264.7; macrophage; GLUCOSIDES; OXYGEN; ANTIOXIDANT; PLANTS; ASSAY;

D O I：

10.1016/j.fitote.2011.08.002

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The in vivo and in vitro mechanistic anti-inflammatory actions of cucurbitacin E (CE) (Citrullus lanatus var. citroides) were examined. The results showed that LPS/INF-gamma increased NO production in RAW264.7 macrophages, whereas L-NAME and CE curtailed it. CE did not reveal any cytotoxicity on RAW264.7 and WRL-68 cells. CE inhibited both COX enzymes with more selectivity toward COX-2. Intraperitoneal injection of CE significantly suppressed carrageenan-induced rat's paw edema. ORAC and FRAP assays showed that CE is not a potent ROS scavenger. It could be concluded that CE is potentially useful in treating inflammation through the inhibition of COX and RNS but not ROS. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1190 / 1197

页数：8

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