Poplar maintains zinc homeostasis with heavy metal genes HMA4 and PCS1

被引:38
作者
Adams, Joshua P. [1 ]
Adeli, Ardeshir [2 ]
Hsu, Chuan-Yu [1 ]
Harkess, Richard L. [3 ]
Page, Grier P. [4 ]
dePamphilis, Claude W. [5 ]
Schultz, Emily B. [1 ]
Yuceer, Cetin [1 ]
机构
[1] Mississippi State Univ, Dept Forestry, Mississippi State, MS 39762 USA
[2] USDA ARS, Mississippi State, MS 39762 USA
[3] Mississippi State Univ, Dept Plant & Soil Sci, Mississippi State, MS 39762 USA
[4] RTI Int, Atlanta, GA 30341 USA
[5] Penn State Univ, Dept Biol, University Pk, PA 16802 USA
关键词
Heavy metal; heavy metal transporter; phytochelatin synthase; poplar nutrition; PHYTOCHELATIN SYNTHASE; THLASPI-CAERULESCENS; NICKEL TOLERANCE; ARABIDOPSIS; CADMIUM; HYPERACCUMULATOR; GLUTATHIONE; EXPRESSION; PLANTS; ATPASE;
D O I
10.1093/jxb/err025
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Perennial woody species, such as poplar (Populus spp.) must acquire necessary heavy metals like zinc (Zn) while avoiding potential toxicity. Poplar contains genes with sequence homology to genes HMA4 and PCS1 from other species which are involved in heavy metal regulation. While basic genomic conservation exists, poplar does not have a hyperaccumulating phenotype. Poplar has a common indicator phenotype in which heavy metal accumulation is proportional to environmental concentrations but excesses are prevented. Phenotype is partly affected by regulation of HMA4 and PCS1 transcriptional abundance. Wild-type poplar down-regulates several transcripts in its Zn-interacting pathway at high Zn levels. Also, overexpressed PtHMA4 and PtPCS1 genes result in varying Zn phenotypes in poplar; specifically, there is a doubling of Zn accumulation in leaf tissues in an overexpressed PtPCS1 line. The genomic complement and regulation of poplar highlighted in this study supports a role of HMA4 and PCS1 in Zn regulation dictating its phenotype. These genes can be altered in poplar to change its interaction with Zn. However, other poplar genes in the surrounding pathway may maintain the phenotype by inhibiting drastic changes in heavy metal accumulation with a single gene transformation.
引用
收藏
页码:3737 / 3752
页数:16
相关论文
共 79 条
  • [1] The Arabidopsis heavy metal P-type ATPase HMA5 interacts with metallochaperones and functions in copper detoxification of roots
    Andrés-Colás, N
    Sancenón, V
    Rodríguez-Navarro, S
    Mayo, S
    Thiele, DJ
    Ecker, JR
    Puig, S
    Peñarrubia, L
    [J]. PLANT JOURNAL, 2006, 45 (02) : 225 - 236
  • [3] Cross-species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleri
    Becher, M
    Talke, IN
    Krall, L
    Krämer, U
    [J]. PLANT JOURNAL, 2004, 37 (02) : 251 - 268
  • [4] A novel CPx-ATPase from the cadmium hyperaccumulator Thlaspi caerulescens
    Bernard, C
    Roosens, N
    Czernic, P
    Lebrun, M
    Verbruggen, N
    [J]. FEBS LETTERS, 2004, 569 (1-3): : 140 - 148
  • [5] Enhanced arsenate reduction by a CDC25-like tyrosine phosphatase explains increased phytochelatin accumulation in arsenate-tolerant Holcus lanatus
    Bleeker, PM
    Hakvoort, HWJ
    Bliek, M
    Souer, E
    Schat, H
    [J]. PLANT JOURNAL, 2006, 45 (06) : 917 - 929
  • [6] Function of phytochelatin synthase in catabolism of glutathione-conjugates
    Blum, Ralph
    Beck, Andreas
    Korte, Arthur
    Stengel, Anna
    Letzel, Thomas
    Lendzian, Klaus
    Grill, Erwin
    [J]. PLANT JOURNAL, 2007, 49 (04) : 740 - 749
  • [7] BRAG H, 2006, PHYSIOL PLANTARUM, V26, P250
  • [8] ZINC AND CADMIUM UPTAKE BY HYPERACCUMULATOR THLASPI-CAERULESCENS GROWN IN NUTRIENT SOLUTION
    BROWN, SL
    CHANEY, RL
    ANGLE, JS
    BAKER, AJM
    [J]. SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1995, 59 (01) : 125 - 133
  • [9] AtOPT6 transports glutathione derivatives and is induced by primisulfuron
    Cagnac, O
    Bourbouloux, A
    Chakrabarty, D
    Zhang, MY
    Delrot, S
    [J]. PLANT PHYSIOLOGY, 2004, 135 (03) : 1378 - 1387
  • [10] CHANEY LR, 2000, PHYT STAT SCI C BOST