Improving the analysis of fatty acids using a combination of gas chromatography and Ag+ liquid chromatography

被引：0

作者：

Czauderna, M ^{[1
]}

Kowalczyk, J ^{[1
]}

Korniluk, K ^{[1
]}

Wasowska, I ^{[1
]}

机构：

[1] Polish Acad Sci, Kielanowski Inst Anim Physiol & Nutr, PL-05110 Jablonna, Poland

来源：

JOURNAL OF ANIMAL AND FEED SCIENCES | 2005年 / 14卷

关键词：

fatty acids; conjugated dienes; gas-liquid; silver ion chromatography;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A method is proposed for routine determination of fatty acids (FAs), including conjugated fatty acids, in the liver of rats and sheep using gas-liquid chromatography (GLC) and Ag+-HPLC. The proposed procedure includes new methods for saponification carried out at room temperature and extraction of free FAs. The obtained free FAs are methylated in an acid-base catalyzed procedure. Due to simultaneous fractionation of methylated FAs using GLC and Ag+-HPLC, the accuracy and sensitivity of the procedure is improved (especially for conjugated linoleic acids).

引用

页码：563 / 566

页数：4