A simplified pyrazinamidase test for pyrazinamide drug susceptibility in Mycobacterium tuberculosis

被引：5

作者：

Aono, Akio ^{[1
]}

Chikamatsu, Kinuyo ^{[1
]}

Yamada, Hiroyuki ^{[1
]}

Igarashi, Yuriko ^{[1
]}

Murase, Yoshiro ^{[1
]}

Takaki, Akiko ^{[1
]}

Mitarai, Satoshi ^{[1
,2
]}

机构：

[1] Japan AntiTB Assoc, Res Inst TB, Dept Mycobacterium Reference & Res, Kiyose, Japan

[2] Nagasaki Univ, Grad Sch Biomed Sci, Basic Mycobacteriosis, Nagasaki, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2018年 / 154卷

关键词：

Pyrazinamide; Pyrazinamidase; Drug susceptibility; Mycobacterium tuberculosis; PNCA; RESISTANCE; IDENTIFICATION; MUTATIONS;

D O I：

10.1016/j.mimet.2018.09.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We modified Wayne's pyrazinamidase test against Mycobacterium tuberculosis to indirectly measure pyrazinamidase activity via pyrazinoic acid in liquid medium. The modified pyrazinamidase test was easy to perform and its results were in complete agreement with those of the conventional Wayne's method, highlighting its potential applicatlon in phenotypic pyrazinamide susceptibility testing.

引用

页码：52 / 54

页数：3

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