Determination of natural and synthetic estrogens in water by gas chromatography with mass spectrometric detection

被引：172

作者：

Quintana, JB ^{[1
]}

Carpinteiro, J ^{[1
]}

Rodríguez, I ^{[1
]}

Lorenzo, RA ^{[1
]}

Carro, AM ^{[1
]}

Cela, R ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Dept Quim Analit Nutr & Bromatol, Santiago De Compostela 15708, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1024卷 / 1-2期

关键词：

water analysis; derivatization; GC; environmental analysis; estrogens; steroids;

D O I：

10.1016/j.chroma.2003.10.074

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the determination of six natural and synthetic estrogens (diethylstilbestrol, estrone, 17beta-estradiol, mestranol, 17alpha-ethinylestradiol and estriol) in water samples is described. Samples, up to 2000 ml, were concentrated using Oasis HLB solid-phase extraction cartridges. Analytes were derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide and determined by GC-MS or GC-MS-MS. The reactivity of several silylation reagents versus aliphatic and aromatic hydroxyl groups contained in the structure of the selected analytes was evaluated. Influence of parameters such as sample pH, nature of the water samples and derivatization conditions on the performance of the whole analytical procedure was systematically studied. Under optimal conditions, quantification limits between 1 and 3 ng/l were achieved for the determination of the considered estrogens in sewage water. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：177 / 185

页数：9

共 21 条

[1] On-line derivatisation in combined high-performance liquid chromatography gas chromatography mass spectrometry [J].