In Vivo Three-dimensional Doppler Variance Imaging of Tumor Angiogenesis on Chorioallantoic Membrane

被引：2

作者：

Qi, Wenjuan ^{[1
]}

Liu, Gangjun ^{[1
]}

Chen, Zhongping ^{[1
]}

机构：

[1] Univ Calif Irvine, Beckman Laser Inst, Irvine, CA 92612 USA

来源：

DYNAMICS AND FLUCTUATIONS IN BIOMEDICAL PHOTONICS VIII | 2011年 / 7898卷

关键词：

Doppler variance; Optical Coherence Tomography; standard deviation; tumor; microvasculature; angiogenesis; OPTICAL DOPPLER; TOMOGRAPHY; MICROENVIRONMENT; RESOLUTION; FLOW;

D O I：

10.1117/12.875186

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Non-invasive tumor microvasculature visualization and characterization play significant roles in the detection of tumors and importantly, for aiding in the development of therapeutic strategies. The feasibility and effectiveness of a Doppler variance standard deviation imaging method for tumor angiogenesis on chorioallantoic membrane were tested in vivo on a rat glioma F98 tumor spheroid. Utilizing a high resolution Doppler Variance Optical Coherence Tomography (DVOCT) system with A-line rate of 20 kHz, three-dimensional mapping of a tumor with a total area of 3x2.5mm(2) was completed within 15 seconds. The top-view image clearly visualized the complex vascular perfusion with the detection of capillaries as small as approximately 10 mu m. The results of the current study demonstrate the capability of the Doppler variance standard deviation imaging method as a non-invasive assessment of tumor angiogenesis, with the potential for its use in clinical settings.

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