Gut microbiota of red swamp crayfish Procambarus clarkii in integrated crayfish-rice cultivation model

被引:69
作者
Shui, Yan [1 ]
Guan, Zheng-Bing [2 ]
Liu, Guo-Feng [1 ,3 ]
Fan, Li-Min [1 ]
机构
[1] Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, 9 Shanshui East Rd, Wuxi 214081, Jiangsu, Peoples R China
[2] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China
[3] Nanjing Agr Univ, Wuxi Fisheries Coll, Nanjing, Peoples R China
基金
中国国家自然科学基金;
关键词
Integrated crayfish-rice cultivation model; Gut microbiota; Procambarus clarkii; Illumina MiSeq sequencing; COMMUNITY; DATABASE; SYSTEM;
D O I
10.1186/s13568-019-0944-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Increasing evidences suggest that intestinal microbiota balance closely correlated with host's health status could affected by external environment. Integrated crayfish-rice cultivation model is a highly efficient artificial ecosystem widely practiced in subtropical China. Less information is available to estimate the influence response to the micro-ecology of crayfish intestine and so as to influence the biological processes. Thus, 16S rRNA high-throughput sequencing approach was employed to investigate the composition diversity and functions of bacterial community in the intestines of Procambarus clarkii farmed within this model. Results exhibited the highly diversity of microflora with dominant phyla Actinobacteria, Proteobacteria, Tenericutes, Firmicutes and Bacteroidetes. The genera of Candidatus Bacilloplasma and Ornithinibacter were presented as predominant population much exceeds in richness comparing to that of other genus. Despite the highly diversity in the bacterial community, the predicted functions indicated relative consistent in biological processing pathway. Collectively, significant richness of genes was observed involved in amino acid and carbohydrate metabolism and membrane transport processing. This study would contribute to the understanding of the impact of growth conditions on host-microbiota relation especially in aquatic animals.
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页数:11
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