Investigating the kinetics of DNA-DNA and PNA-DNA interactions using surface plasmon resonance-enhanced fluorescence spectroscopy

被引：74
作者：
Kambhampati, D
Nielsen, PE
Knoll, W
机构：
[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany
[2] Panum Inst, Ctr Biomol Recognit, Dept Med Biochem & Genet, DK-2200 Copenhagen, Denmark
来源：
BIOSENSORS & BIOELECTRONICS | 2001年 / 16卷 / 9-12期
关键词：
peptide nucleic acid (PNA); DNA; plasmon;
D O I：
10.1016/S0956-5663(01)00239-1
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Plasmon surface polaritons, resonantly excited in the Kretschmann format, are used to enhance the fluorescence emission of chromophore-labeled oligonucleotides (15mers) binding to surface-attached (via biotin-streptavidin linkages) complement catcher probes. A detailed analysis of the association and dissociation kinetics as well as the affinity constants is given for a mismatch I hybrid, emphasizing, in particular, the experimental conditions that are required to allow for an artifact-free determination of rate constants. A first comparison between DNA- and peptide nucleic acid (PNA-) probes shows similar affinities, however, significant deviations from single-exponential kinetics predicted by a simple Langmuir model for the PNA case are found. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：1109 / 1118
页数：10
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