Isolation and characterization of two dinucleotide and four tetranucleotide polymorphic microsatellite loci in the Iberian midwife toad Alytes cistemasii

被引：0
|
作者：
Gonçalves, H
Pereira, R
Rowe, G
Beebee, T
Ferrand, N
机构：
[1] CIBIO, Ctr Invest Biodivers & Recursos Genet, P-4485661 Vairao, Portugal
[2] Univ Porto, Fac Ciencias, Dept Zool & Antropol, P-4099002 Oporto, Portugal
[3] Univ Sussex, Sch Life Sci, Brighton BN1 9QG, E Sussex, England
来源：
MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 04期
关键词：
Alytes cisternasii; microsatellite; midwife toad; polymorphism;
D O I：
10.1111/j.1471-8286.2005.01057.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Iberian midwife toad Alytes cisternasii is an endemic species of the southwestern and central parts of the Iberian Peninsula. Allozyme analysis of Iberian midwife toads indicated no inter- or intrapopulation variation and a more sensitive method is required to quantify genetic differentiation and gene flow between populations of this species. We describe here the development of primers for the amplification by the polymerase chain reaction (PCR) of six polymorphic microsatellite loci in A. cisternasii.
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收藏
页码：767 / 769
页数：3
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