Crystal structure of a single-chain trimer of human adiponectin globular domain

被引：38
作者：
Min, Xiaoshan [1 ]
Lemon, Bryan [2 ]
Tang, Jie [2 ]
Liu, Qiang [2 ]
Zhang, Richard [2 ]
Walker, Nigel [1 ]
Li, Yang [3 ]
Wang, Zhulun [1 ]
机构：
[1] Amgen Inc, Dept Mol Struct, 1120 Vet Blvd, San Francisco, CA 94080 USA
[2] Amgen Inc, Dept Prot Sci, San Francisco, CA 94080 USA
[3] Amgen Inc, Dept Metab Disorders, San Francisco, CA 94080 USA
来源：
FEBS LETTERS | 2012年 / 586卷 / 06期
关键词：
X-ray crystal structure; Adiponectin; Single-chain globular domain; Calcium binding; COMPLEMENT-RELATED PROTEIN; INSULIN-RESISTANCE; ADIPOSE; EXPRESSION; OBESITY; MICE; C1Q;
D O I：
10.1016/j.febslet.2012.02.024
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Adiponectin is increasingly recognized as a potential therapeutic agent for the treatment of diabetes and other metabolic diseases. It circulates in plasma as homotrimers and higher-order oliogomers of homotrimers. To facilitate the production of active recombinant adiponectin as a therapeutic tool, we designed a single-chain globular domain adiponectin (sc-gAd) in which three monomer sequences are linked together in tandem to form one contiguous polypeptide. Here, we present the crystal structure of human sc-gAd at 2.0 angstrom resolution. The structure reveals a similar trimeric topology to that of mouse gAd protein. Trimer formation is further rigidified by three calcium ions. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：912 / 917
页数：6
相关论文
共 33 条
  • [1] Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity
    Arita, Y
    Kihara, S
    Ouchi, N
    Takahashi, M
    Maeda, K
    Miyagawa, J
    Hotta, K
    Shimomura, I
    Nakamura, T
    Miyaoka, K
    Kuriyama, H
    Nishida, M
    Yamashita, S
    Okubo, K
    Matsubara, K
    Muraguchi, M
    Ohmoto, Y
    Funahashi, T
    Matsuzawa, Y
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 257 (01) : 79 - 83
  • [2] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY
    BAILEY, S
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 : 760 - 763
  • [3] Banga Anannya, 2008, Metabolic Syndrome and Related Disorders, V6, P103, DOI 10.1089/met.2007.0033
  • [4] Insight into Schmid metaphyseal chondrodysplasia from the crystal structure of the collagen X NC1 domain trimer
    Bogin, O
    Kvansakul, M
    Rom, E
    Singer, J
    Yayon, A
    Hohenester, E
    [J]. STRUCTURE, 2002, 10 (02) : 165 - 173
  • [5] A transgenic mouse with a deletion in the collagenous domain of adiponectin displays elevated circulating adiponectin and improved insulin sensitivity
    Combs, TP
    Pajvani, UB
    Berg, AH
    Lin, Y
    Jelicks, LA
    Laplante, M
    Nawrocki, AR
    Rajala, MW
    Parlow, AF
    Cheeseboro, L
    Ding, YY
    Russell, RG
    Lindemann, D
    Hartley, A
    Baker, GRC
    Obici, S
    Deshaies, Y
    Ludgate, M
    Rossetti, L
    Scherer, PE
    [J]. ENDOCRINOLOGY, 2004, 145 (01) : 367 - 383
  • [6] Coot:: model-building tools for molecular graphics
    Emsley, P
    Cowtan, K
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 2126 - 2132
  • [7] Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice
    Fruebis, J
    Tsao, TS
    Javorschi, S
    Ebbets-Reed, D
    Erickson, MRS
    Yen, FT
    Bihain, BE
    Lodish, HF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (04) : 2005 - 2010
  • [8] The crystal structure of the globular head of complement protein C1q provides a basis for its versatile recognition properties
    Gaboriaud, C
    Juanhuix, J
    Gruez, A
    Lacroix, M
    Darnault, C
    Pignol, D
    Verger, D
    Fontecilla-Camps, JC
    Arlaud, GJ
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (47) : 46974 - 46982
  • [9] Generation of Novel Long-Acting Globular Adiponectin Molecules
    Ge, Hongfei
    Xiong, Yumei
    Lemon, Bryan
    Lee, Ki Jeong
    Tang, Jay
    Wang, Ping
    Weiszmann, Jennifer
    Hawkins, Nessa
    Laudemann, John
    Min, Xiaoshan
    Penny, David
    Wolfe, Tom
    Liu, Qiang
    Zhang, Richard
    Yeh, Weh-Chen
    Shen, Wenyan
    Lindberg, Richard
    Wang, Zhulun
    Sheng, Jackie
    Li, Yang
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2010, 399 (01) : 113 - 119
  • [10] Small revisions to predicted distances around metal sites in proteins
    Harding, MM
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2006, 62 : 678 - 682
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