Heliorhodopsin Helps Photolyase to Enhance the DNA Repair Capacity

被引：19

作者：

Shim, Jin-gon ^{[1
]}

Cho, Shin-Gyu ^{[1
,2
]}

Kim, Se-Hwan ^{[1
]}

Chuon, Kimleng ^{[1
]}

Meas, Seanghun ^{[1
]}

Choi, Ahreum ^{[3
]}

Jung, Kwang-Hwan ^{[1
]}

机构：

[1] Sogang Univ, Dept Life Sci, Seoul, South Korea

[2] Sogang Univ, Res Inst Basic Sci, Seoul, South Korea

[3] Natl Inst Ecol, Res Ctr Endangered Species, Yeongyang Gun, Gyeongsangbuk D, South Korea

来源：

MICROBIOLOGY SPECTRUM | 2022年 / 10卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

DNA repair; photolyase; microbial rhodopsin; protein-protein interaction; heliorhodopsin; evolution; CLASS-II DNA; RHODOPSIN; BINDING; PHOTOTROPHY; TRANSDUCER; MECHANISMS; FAD;

D O I：

10.1128/spectrum.02215-22

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Light quality is a significant factor for living organisms that have photosensory systems, such as rhodopsin, a seven alpha-helical transmembrane protein with the retinal chromophore. Here, we report, for the first time, the function of new rhodopsin, which is an inverted 7-transmembrane protein, isolated from Trichococcus flocculiformis. T. flocculiformis heliorhodopsin (TfHeR) works as a regulatory helper rhodopsin that binds with class 2 cyclobutane pyrimidine dimer (CPDII) photolyase to broaden the spectrum and upregulate DNA repair activity. We have confirmed their interaction through isothermal titration calorimetry (dissociation constant of 21.7 mu M) and identified the charged residues for the interaction. Based on in vivo and in vitro experiments, we showed that the binding of heliorhodopsin with photolyase improved photolyase activity by about 3-fold to repair UV-caused DNA damage. Also, the DNA repair activity of TfHeR/T. flocculiformis photolyase (TfPHR) was observed in the presence of green light. Our results suggested that heliorhodopsin directly controls the activity of photolyase and coevolves to broaden the activity spectrum by protein-protein interaction.

引用

页数：11

共 32 条

[11] Zinc Binding to Heliorhodopsin [J].