Neurotoxicity Induced by Okadaic Acid in the Human Neuroblastoma SH-SY5Y Line Can Be Differentially Prevented by α7 and β2*Nicotinic Stimulation

被引:43
作者
del Barrio, Laura [2 ,3 ]
Dolores Martin-de-Saavedra, Maria [2 ,4 ]
Romero, Alejandro [2 ,5 ]
Parada, Esther [2 ]
Egea, Javier [2 ,4 ]
Avila, Jesus [6 ]
McIntosh, John Michael [7 ]
Wonnacott, Susan [3 ]
Lopez, Manuela G. [1 ,2 ,4 ]
机构
[1] Univ Autonoma Madrid, Fac Med, Dept Farmacol, Inst Teofilo Hernando, E-28029 Madrid, Spain
[2] Univ Autonoma Madrid, Fac Med, Dept Farmacol & Terapeut, E-28029 Madrid, Spain
[3] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England
[4] Hosp La Paz, Res Inst IdiPaz, Madrid 28029, Spain
[5] Univ Complutense Madrid, Fac Vet Med, Dept Toxicol & Pharmacol, E-28040 Madrid, Spain
[6] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain
[7] Univ Utah, Dept Biol & Psychiat, Salt Lake City, UT 84112 USA
关键词
okadaic acid; SH-SY5Y neuroblastoma; nicotinic receptors; hyperphosphorylation of tau; PNU; 282987; 5IA; 85380; NICOTINIC ACETYLCHOLINE-RECEPTORS; GLYCOGEN-SYNTHASE KINASE-3-BETA; ABNORMALLY PHOSPHORYLATED-TAU; ALZHEIMER-DISEASE BRAIN; PROTEIN-KINASE; CELL-LINE; TYROSINE PHOSPHORYLATION; PARKINSONS-DISEASE; FIBRILLAR POLYMERS; OXIDATIVE STRESS;
D O I
10.1093/toxsci/kfr163
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
A good model of neuronal death that reproduces the characteristic tau (tau) hyperphosphorylation of Alzheimers disease is the use of okadaic acid (OA). The aim of this study was to determine the contribution of alpha 7 and beta 2* nicotinic acetylcholine receptor (nAChR) subtypes to neuroprotection against OA in the SH-SY5Y cell line by using the selective alpha 7 and beta 2* nAChR agonists PNU 282987 and 5-Iodo-A85380, respectively. The results of this study show that both alpha 7 and beta 2* nAChR can afford neuroprotection against OA-induced neurotoxicity. Protection mediated by alpha 7 nAChRs was independent of Ca2+ and involved the intracellular signaling pathway Janus Kinase-2/Phosphatidylinositol-3-kinase/Akt. When Ca2+ entry was promoted through the alpha 7 nAChR by using the alpha 7-selective positive allosteric modulator PNU 120596, protection was lost. By contrast, protection mediated by beta 2* nAChRs was Ca2+ dependent and implicated the signaling pathways PI3K/Akt and extracellular regulated kinase 1/2. Both alpha 7 and beta 2* nAChR activation converged on downregulation of GSK-3 beta and reduction of tau phosphorylation in cells undergoing cell death induced by OA. Therefore, targeting nAChR could offer a strategy for reducing neurodegeneration secondary to hyperphosphorylation of protein tau.
引用
收藏
页码:193 / 205
页数:13
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