CAC2634-disrupted mutant of Clostridium acetobutylicum can be electrotransformed in air

被引：7

作者：

Dong, H. ^{[1
]}

Tao, W. ^{[1
]}

Zhu, L. ^{[1
]}

Zhang, Y. ^{[1
]}

Li, Y. ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2011年 / 53卷 / 03期

关键词：

CAC2634; Clostridium acetobutylicum; electrotransformation in air; simplified electrotransformation process; METABOLIC NETWORK; ATCC; 824; GENES; DNA; INACTIVATION; TOLERANCE; BUTANOL; PATHWAY; STRAIN;

D O I：

10.1111/j.1472-765X.2011.03111.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming. Methods and Results: The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously constructed Restriction-Modification system deficient Cl. acetobutylicum mutant SMB009 was disrupted using ClosTron method. The resulted mutant SMB012 can be electrotransformed in air with an efficiency of 1.2-3.1 x 10(3) transformants mu g(-1) DNA. Conclusions: We demonstrated that the disruption of CAC2634 in Cl. acetobutylicum enables its electrotransformation in air. Significance and Impact of the Study: The electrotransformation process of Cl. acetobutylicum could be significantly simplified, especially when operating multiple electrotransformations.

引用

页码：379 / 382

页数：4

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