HPLC does not differentiate Mycobacterium paratuberculosis from Mycobacterium avium

被引：5
作者：
Dei, R
Tortoli, E
Bartoloni, A
Simonetti, MT
Lillini, E
机构：
[1] Univ Florence, Ist Microbiol, I-50134 Florence, Italy
[2] Osped Careggi, Lab Microbiol & Virol, Florence, Italy
[3] Univ Florence, Ist Malattie Infettive, Florence, Italy
[4] Ist Zooprofilattico Sperimentale Reg Lazio & Tosc, Rome, Italy
来源：
VETERINARY MICROBIOLOGY | 1999年 / 65卷 / 03期
关键词：
Mycobacterium paratuberculosis; diagnosis-bacteria; HPLC;
D O I：
10.1016/S0378-1135(98)00292-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
HPLC, which is gaining its place as identification tool in mycobacteriology laboratories, has been proposed to distinguish Mycobacterium paratuberculosis from Mycobacterium avium. We had reported no significant difference between M. avium and M. paratuberculosis reference strain ATCC 19698, Because of the advantages offered by such a method, we enlarged our observations to include more isolates of M. paratuberculosis. Within the double cluster of peaks obtained by both M. avium and M. paratuberculosis, we could not find a consistent difference typical of M. paratuberculosis. Therefore, the present study confirmed that M. avium and M. paratuberculosis could not be distinguished by HPLC, raising doubts of a straightforward use of HPLC to identify M. paratuberculosis. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：209 / 213
页数：5
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