Isolation and culture of colon cancer stem cells

被引：80

作者：

Cammareri, Patrizia ^{[1
]}

Lombardo, Ylenia ^{[2
]}

Francipane, Maria Giovanna ^{[2
]}

Bonventre, Sebastino ^{[2
]}

Todaro, Matilde ^{[2
]}

Stassit, Giorgio ^{[2
]}

机构：

[1] Univ Palermo, Dept GENURTO, I-90127 Palermo, Italy

[2] Univ Palermo, Dept Surg & Oncol Sci, I-90127 Palermo, Italy

来源：

STEM CELL CULTURE | 2008年 / 86卷

关键词：

D O I：

10.1016/S0091-679X(08)00014-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cancer stem cells (CSCs) resemble normal stem cells in several ways. Both cell types are self-renewing and when they divide, one of the daughter cells differentiates while the other retains stem cell properties, including the ability to divide in the same way again. CSCs have been demonstrated to exist in several solid tumors, including colon carcinoma; these cells are able to initiate and sustain tumor growth. There are essentially three different methods to isolate CSCs: establishment culture, the MACS (magnetic cell sorting) technology, and the FACS (fluorescence-activated cell sorting) technology.

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页码：311 / +

页数：16

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