Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

被引：43

作者：

Sharei, Armon ^{[1
,4
,5
]}

Trifonova, Radiana ^{[6
]}

Jhunjhunwala, Siddharth ^{[5
]}

Hartoularos, George C. ^{[1
]}

Eyerman, Alexandra T. ^{[1
]}

Lytton-Jean, Abigail ^{[5
]}

Angin, Mathieu ^{[2
,3
]}

Sharma, Siddhartha ^{[2
,3
]}

Poceviciute, Roberta ^{[1
]}

Mao, Shirley ^{[1
]}

Heimann, Megan ^{[1
]}

Liu, Sophia ^{[1
]}

Talkar, Tanya ^{[1
]}

Khan, Omar F. ^{[5
]}

Addo, Marylyn ^{[2
,3
]}

von Andrian, Ulrich H. ^{[2
,3
,4
]}

Anderson, Daniel G. ^{[1
,5
]}

Langer, Robert ^{[1
,5
]}

Lieberman, Judy ^{[6
]}

Jensen, Klavs F. ^{[1
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[2] MIT, MGH, Ragon Inst, Cambridge, MA 02139 USA

[3] Harvard Univ, Cambridge, MA 02138 USA

[4] Harvard Univ, Sch Med, Dept Microbiol & Immunobiol, Boston, MA USA

[5] MIT, Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA

[6] Harvard Univ, Sch Med, Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02163 USA

来源：

PLOS ONE | 2015年 / 10卷 / 04期

基金：

美国国家卫生研究院;

关键词：

APTAMER-SIRNA CHIMERAS; SMALL INTERFERING RNAS; INTRACELLULAR DELIVERY; DENDRITIC CELLS; T-CELLS; ELECTROPORATION; INFECTION; NANOPARTICLES; PEPTIDES; PROTEINS;

D O I：

10.1371/journal.pone.0118803

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach's potential to engineer cell function is demonstrated in HIV infection studies.

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页数：12

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