Aromatase immunolocalization in human ductuli efferentes and proximal ductus epididymis

被引：50
作者：
Carpino, A [1 ]
Romeo, F
Rago, V
机构：
[1] Univ Calabria, Dipartimento Biol Cellulare, Fac Pharm, I-87030 Arcavacata Di Rende, Italy
[2] Annunziata Hosp, Anat Pathol Unit, Consenza, Italy
来源：
JOURNAL OF ANATOMY | 2004年 / 204卷 / 03期
关键词：
caput epididymis; oestrogens; human genital tract; male reproduction; P450arom;
D O I：
10.1111/j.0021-8782.2004.00272.x
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Cytochrome P450 aromatase is a terminal enzyme that catalyses the conversion of androgens into oestrogens. This study investigated the immunohistochemical localization of aromatase in human efferent ductules and proximal ductus epididymis using a mouse anti-human monoclonal P450arom IgG as primary antibody and a goat anti-mouse biotinylated IgG as secondary antibody. A strong immunoreaction was observed in the epithelial cell cytoplasm of both ductuli efferentes and proximal ductus epididymis, whereas the smooth muscle cells were immunonegative in the two regions. The results show, for the first time in humans, that epithelial cells of ductuli efferentes and proximal caput epididymis express aromatase, suggesting that locally produced oestrogens may have a role in epididymal function.
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收藏
页码：217 / 220
页数：4
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