Investigation of Red Blood Cells by Atomic Force Microscopy

被引：21

作者：

Sergunova, Viktoria ^{[1
]}

Leesment, Stanislav ^{[2
]}

Kozlov, Aleksandr ^{[3
]}

Inozemtsev, Vladimir ^{[1
]}

Platitsina, Polina ^{[4
]}

Lyapunova, Snezhanna ^{[1
]}

Onufrievich, Alexander ^{[5
]}

Polyakov, Vyacheslav ^{[2
]}

Sherstyukova, Ekaterina ^{[1
,3
]}

机构：

[1] VA Negovsky Res Inst Gen Reanimatol, Fed Res & Clin Ctr Intens Care Med & Rehabilitol, Lab Biophys Cell Membranes Crit State, Moscow 107031, Russia

[2] NT MDT Spectrum Instruments, Proezd 4922,4-3 Zelenograd, Zelenograd 124460, Russia

[3] Sechenov Univ, Sechenov First Moscow State Med Univ, Dept Med & Biol Phys, Moscow 119991, Russia

[4] Inst Biotechn Syst & Technol Natl Res MIET, Shokin Sq Build1, Zelenograd 124498, Russia

[5] Minist Def Russian Federat, NN Burdenko Main Mil Clin Hosp, Fed State Budgetary Inst, Hosp Sq,Build 3, Moscow 105094, Russia

来源：

SENSORS | 2022年 / 22卷 / 05期

关键词：

imaging; atomic force microscopy; red blood cells; morphology; nanostructure; cytoskeleton; membrane stiffness; ERYTHROCYTE CYTOSKELETON; MEMBRANES; NANOSTRUCTURE; VISUALIZATION; STIFFNESS;

D O I：

10.3390/s22052055

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Currently, much research is devoted to the study of biological objects using atomic force microscopy (AFM). This method's resolution is superior to the other non-scanning techniques. Our study aims to further emphasize some of the advantages of using AFM as a clinical screening tool. The study focused on red blood cells exposed to various physical and chemical factors, namely hemin, zinc ions, and long-term storage. AFM was used to investigate the morphological, nanostructural, cytoskeletal, and mechanical properties of red blood cells (RBCs). Based on experimental data, a set of important biomarkers determining the status of blood cells have been identified.

引用

页数：13

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