Methodologies to Isolate and Purify Clinical Grade Extracellular Vesicles for Medical Applications

被引：78

作者：

Akbar, Asma ^{[1
]}

Malekian, Farzaneh ^{[1
]}

Baghban, Neda ^{[1
]}

Kodam, Sai Priyanka ^{[1
]}

Ullah, Mujib ^{[1
,2
,3
]}

机构：

[1] Stanford Univ, Sch Med, Inst Immun & Transplantat Stem Cell Biol & Regene, Palo Alto, CA 94304 USA

[2] Genentech Inc, Dept Canc Immunol, San Francisco, CA 94080 USA

[3] Stanford Univ, Mol Med Dept Med, Palo Alto, CA 94304 USA

来源：

CELLS | 2022年 / 11卷 / 02期

关键词：

extracellular vesicles; stem cells; isolation and purification methods; clinical application; MESENCHYMAL STROMAL CELLS; EXOSOME-MEDIATED DELIVERY; CANCER-THERAPY; ALZHEIMERS-DISEASE; BLOOD EXOSOMES; MESSENGER-RNA; STEM-CELLS; FAS LIGAND; TNF-ALPHA; BIOMARKERS;

D O I：

10.3390/cells11020186

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The use of extracellular vesicles (EV) in nano drug delivery has been demonstrated in many previous studies. In this study, we discuss the sources of extracellular vesicles, including plant, salivary and urinary sources which are easily available but less sought after compared with blood and tissue. Extensive research in the past decade has established that the breadth of EV applications is wide. However, the efforts on standardizing the isolation and purification methods have not brought us to a point that can match the potential of extracellular vesicles for clinical use. The standardization can open doors for many researchers and clinicians alike to experiment with the proposed clinical uses with lesser concerns regarding untraceable side effects. It can make it easier to identify the mechanism of therapeutic benefits and to track the mechanism of any unforeseen effects observed.

引用

页数：22

共 218 条

[1] First trimester trophoblast cells secrete Fas ligand which induces immune cell apoptosis [J].