Dual-wavelength digital holographic phase and fluorescence microscopy combining with Raman spectroscopy for micro-quartz pieces-based dual-channel encoded suspension array

被引：3

作者：

Chen, Xuejing ^{[1
,2
]}

Shen, Zhiyuan ^{[1
,3
]}

He, Yonghong ^{[1
,2
]}

Guan, Tian ^{[1
,4
]}

He, Qinghua ^{[1
,2
]}

Wang, Bei ^{[1
]}

Xie, Luyuan ^{[1
,4
]}

Feng, Guangxia ^{[1
,4
]}

Lu, Bangrong ^{[1
]}

Zhou, Xuesi ^{[1
]}

Liu, Jiafan ^{[5
]}

Fan, Zhigang ^{[5
]}

机构：

[1] Tsinghua Univ, Shenzhen Key Lab Minimal Invas Med Technol, Inst Opt Imaging & Sensing, Grad Sch Shenzhen, Shenzhen 518055, Peoples R China

[2] Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China

[3] Orbbec Co Ltd, Shenzhen, Peoples R China

[4] Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China

[5] Sun Yat Sen Univ, Dept Glaucoma, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Guangdong, Peoples R China

来源：

OPTICS EXPRESS | 2019年 / 27卷 / 03期

基金：

美国国家科学基金会;

关键词：

QUANTUM-DOT BARCODES; REAL-TIME; NANOPARTICLES; MONOLAYERS; DYNAMICS; BEADS;

D O I：

10.1364/OE.27.001894

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Dual-wavelength digital holographic phase and fluorescence microscopy (DW-DHPFM), combining with Raman spectroscopy, is designed to achieve the detection and analysis of biomolecules with a new dual-channel encoding method. This employs the Raman reporter molecules assembled micro-quartz pieces (MQPs) as microcarriers of suspension array (SA). The dual-wavelength digital holographic phase microscopy (DW-DHPM) and Raman spectroscopy are served as the decoding platforms. and the fluorescence microscopy is used to quantify target analytes. Considering the independence between encoding and label signal, the above two encoding channels could effectively avoid the crosstalk in immunoassay process, and the combination of two encoding methods expand the encoding capacity with a considerable magnitude. Accurate and stable decoding abilities are verified by multiplexed immunoassay experiment and the quantitative analysis of targets with high-sensitivity is confirmed by concentration gradient experiments. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：1894 / 1910

页数：17

共 46 条

[1] Single-shot, dual-wavelength digital holography based on polarizing separation [J].

Abdelsalam, D. G. ;

Magnusson, Robert ;

Kim, Daesuk .

APPLIED OPTICS, 2011, 50 (19) :3360-3368

[2] Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy [J].

Boss, Daniel ;

Kuehn, Jonas ;

Jourdain, Pascal ;

Depeursinge, Christian ;

Magistretti, Pierre J. ;

Marquet, Pierre .

JOURNAL OF BIOMEDICAL OPTICS, 2013, 18 (03)

[3] Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection [J].

Cao, YWC ;

Jin, RC ;

Mirkin, CA .

SCIENCE, 2002, 297 (5586) :1536-1540

[4] Parameter-optimized digital holographic microscope for high-resolution living-cell analysis [J].

Carl, D ;

Kemper, B ;

Wernicke, G ;

von Bally, G .

APPLIED OPTICS, 2004, 43 (36) :6536-6544

[5] Multiplexed Sensing of Ions with Barcoded Polyelectrolyte Capsules [J].

del Mercato, Loretta L. ;

Abbasi, Azhar Z. ;

Ochs, Markus ;

Parak, Wolfgang J. .

ACS NANO, 2011, 5 (12) :9668-9674

[6] Synergism between particle-based multiplexing and microfluidics technologies may bring diagnostics closer to the patient [J].

Derveaux, S. ;

Stubbe, B. G. ;

Braeckmans, K. ;

Roelant, C. ;

Sato, K. ;

Demeester, J. ;

De Smedt, S. C. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 391 (07) :2453-2467

[7]

Dunbar Sherry A., 2007, V394, P1, DOI 10.1007/978-1-59745-512-1_1

[8] SELF-ASSEMBLED METAL COLLOID MONOLAYERS - AN APPROACH TO SERS SUBSTRATES [J].

FREEMAN, RG ;

GRABAR, KC ;

ALLISON, KJ ;

BRIGHT, RM ;

DAVIS, JA ;

GUTHRIE, AP ;

HOMMER, MB ;

JACKSON, MA ;

SMITH, PC ;

WALTER, DG ;

NATAN, MJ .

SCIENCE, 1995, 267 (5204) :1629-1632

[9] Quantum dot-encoded mesoporous beads with high brightness and uniformity: Rapid readout using flow cytometry [J].

Gao, XH ;

Nie, SM .

ANALYTICAL CHEMISTRY, 2004, 76 (08) :2406-2410

[10] Phase imaging without 2π ambiguity by multiwavelength digital holography [J].

Gass, J ;

Dakoff, A ;

Kim, MK .

OPTICS LETTERS, 2003, 28 (13) :1141-1143

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