Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers

被引：14

作者：

Yuan, Wang Jun ^{[1
,2
]}

Han, Yuan Ji ^{[1
]}

Dong, Mei Fang ^{[1
]}

Shang, Fu De ^{[1
]}

机构：

[1] Henan Univ, Coll Life Sci, Key Lab Plant Stress Biol, Kaifeng 475004, Peoples R China

[2] Henan Univ, Inst Chinese Mat Midia, Kaifeng 475004, Peoples R China

来源：

ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2011年 / 14卷 / 01期

关键词：

AFLP marker; cluster analysis; genetic diversity; Osmanthus fragrans; GERMPLASM;

D O I：

10.2225/vol14-issue1-fulltext-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study was conducted to reveal genetic diversity among 100 Osmanthus fragrans cultivars using amplified fragment length polymorphism (AFLP) markers. Eight AFLP primer combinations produced a total of 443 polymorphic fragments with an average of 64 per primer combination. The percentage of polymorphic bands (86.81%), the resolving power (Rp) (32.71) and the PIC values (0.331) showed the efficiency of used primer combinations. The revealed AFLP makers were effective in distinguishing all the cultivars considered. Cluster analysis were performed to assess patterns of diversity among cultivars and showed the abundant genetic diversity. The overall distribution pattern of molecular variation suggested that 93.33% of the total genetic variance was within the identified groups and 6.67% of the genetic variation was among the identified groups. Our results showed that AFLP markers are useful for Osmanthus fragrans germplasm discrimination as well as for investigation of genetic diversity and variation. The information will facilitate germplasm identification, conservation and new cultivar development.

引用

页数：9