Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers

被引：14
作者：
Yuan, Wang Jun [1 ,2 ]
Han, Yuan Ji [1 ]
Dong, Mei Fang [1 ]
Shang, Fu De [1 ]
机构：
[1] Henan Univ, Coll Life Sci, Key Lab Plant Stress Biol, Kaifeng 475004, Peoples R China
[2] Henan Univ, Inst Chinese Mat Midia, Kaifeng 475004, Peoples R China
来源：
ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2011年 / 14卷 / 01期
关键词：
AFLP marker; cluster analysis; genetic diversity; Osmanthus fragrans; GERMPLASM;
D O I：
10.2225/vol14-issue1-fulltext-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This study was conducted to reveal genetic diversity among 100 Osmanthus fragrans cultivars using amplified fragment length polymorphism (AFLP) markers. Eight AFLP primer combinations produced a total of 443 polymorphic fragments with an average of 64 per primer combination. The percentage of polymorphic bands (86.81%), the resolving power (Rp) (32.71) and the PIC values (0.331) showed the efficiency of used primer combinations. The revealed AFLP makers were effective in distinguishing all the cultivars considered. Cluster analysis were performed to assess patterns of diversity among cultivars and showed the abundant genetic diversity. The overall distribution pattern of molecular variation suggested that 93.33% of the total genetic variance was within the identified groups and 6.67% of the genetic variation was among the identified groups. Our results showed that AFLP markers are useful for Osmanthus fragrans germplasm discrimination as well as for investigation of genetic diversity and variation. The information will facilitate germplasm identification, conservation and new cultivar development.
引用
收藏
页数：9
相关论文
共 20 条
[1]   Comparative analysis of genetic diversity in two Tunisian collections of fig cultivars based on random amplified polymorphic DNA and inter simple sequence repeats fingerprints [J].
Amel, SH ;
Khaled, C ;
Messaoud, M ;
Mohamed, M ;
Mokhtar, T .
GENETIC RESOURCES AND CROP EVOLUTION, 2005, 52 (05) :563-573
[2]  
[Anonymous], 1988, NTSYS-PC: Numerical taxonomy and multivariate analysis system
[3]   Molecular characterization of natural hybrids of Phytophthora nicotianae and P-cactorum [J].
Bonants, PJM ;
Hagenaar-de Weerdt, M ;
Man in't Veld, WA ;
Baayen, RP .
PHYTOPATHOLOGY, 2000, 90 (08) :867-874
[4]   Genetic diversity of eggplant (Solanum melongena) germplasm from Turkey assessed by SSR and RAPD markers [J].
Demir, K. ;
Bakir, M. ;
Sarikamis, G. ;
Acunalp, S. .
GENETICS AND MOLECULAR RESEARCH, 2010, 9 (03) :1568-1576
[5]   Analysis of genetic diversity in a sweet potato (Ipomoea batatas L.) germplasm collection from Tanzania as revealed by AFLP [J].
Elameen, Abdelhameed ;
Fjellheim, Siri ;
Larsen, Arild ;
Rognli, Odd Arne ;
Sundheim, Leif ;
Msolla, Susan ;
Masumba, Esther ;
Mtunda, Kiddo ;
Klemsdal, Sonja S. .
GENETIC RESOURCES AND CROP EVOLUTION, 2008, 55 (03) :397-408
[6]   Molecular identification of new varieties of Nierembergia linariaefolia (Graham), a native Argentinean ornamental plant [J].
Escandon, Alejandro S. ;
Zelener, Noga ;
Perez de la Torre, Mariana ;
Soto, Silvina .
JOURNAL OF APPLIED GENETICS, 2007, 48 (02) :115-123
[7]   Genetic relatedness among Tunisian plum cultivars by random amplified polymorphic DNA analysis and evaluation of phenotypic characters [J].
Hend, Ben Tamarzizt ;
Ghada, Baraket ;
Sana, Ben Mustapha ;
Mohamed, Marrakchi ;
Mokhtar, Trifi ;
Amel, Salhi-Hannachi .
SCIENTIA HORTICULTURAE, 2009, 121 (04) :440-446
[8]  
Li Mei Li Mei, 2009, Acta Horticulturae Sinica, V36, P1667
[9]  
LIU Y.L, 1985, J NANJING FORESTRY U, V1, P30
[10]   Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces [J].
Muthusamy, Saraladevi ;
Kanagarajan, Selvaraju ;
Ponnusamy, Shanmugasundaram .
ELECTRONIC JOURNAL OF BIOTECHNOLOGY, 2008, 11 (03)
12