Notes on theory and experimental conditions behind two-photon excitation microscopy

被引：12

作者：

Esposito, A

Federici, F

Usai, C

Cannone, F

Chirico, G

Collini, M

Diaspro, A

机构：

[1] Univ Genoa, Dept Phys, LAMBS, Lab Adv Microscopy, I-16146 Genoa, Italy

[2] Univ Genoa, Dept Phys, Ist Nazl Fis Mat, I-16146 Genoa, Italy

[3] European Neurosci Inst, Cell Biophys Grp, D-37073 Gottingen, Germany

[4] CNR, Inst Biophys, I-16149 Genoa, Italy

[5] Univ Milano Bicocca, Dept Phys, I-20126 Milan, Italy

[6] Univ Milano Bicocca, Lab Adv Biospect, I-20126 Milan, Italy

[7] Univ Milano Bicocca, INFM, I-20126 Milan, Italy

[8] Univ Milano Bicocca, Dept Phys, I-20126 Milan, Italy

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2004年 / 63卷 / 01期

关键词：

two-photon excitation; fluorescence emission; optical sectioning effect;

D O I：

10.1002/jemt.10425

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

This report deals with the fundamental quantum physics behind two-photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission.

引用

页码：12 / 17

页数：6

共 21 条

[11]

Loudon R., 1983, The Quantum Theory of Light

[12]

MASTERS BR, 2002, SPIE MILESTONE SERIE

[13] Molecular photodynamics involved in multi-photon excitation fluorescence microscopy [J].