TRANSPLANTATION OF OLFACTORY ENSHEATHING CELLS PROMOTES AXONAL REGENERATION AND FUNCTIONAL RECOVERY OF PERIPHERAL NERVE LESION IN RATS

被引：26

作者：

Guerout, Nicolas ^{[1
]}

Duclos, Celia ^{[1
]}

Drouot, Laurent ^{[2
]}

Abramovici, Olivia ^{[1
]}

Bon-Mardion, Nicolas ^{[1
,3
]}

Lacoume, Yann ^{[1
]}

Jean, Laetitia ^{[2
]}

Boyer, Olivier ^{[2
]}

Marie, Jean-Paul ^{[1
,3
]}

机构：

[1] Univ Rouen, Expt Surg Lab,Fac Med & Pharm, Grp Rech Handicap Ventilatoire,Inst Med Res, European Inst Peptide Res IFRMP 23,UPRES EA 3830, F-76183 Rouen, France

[2] Univ Rouen, INSERM U905, Fac Med & Pharm, Inst Med Res, F-76183 Rouen, France

[3] Rouen Univ Hosp Charles Nicolle, Dept Otorhinolaryngol Head & Neck Surg, Rouen, France

来源：

MUSCLE & NERVE | 2011年 / 43卷 / 04期

关键词：

axonal regrowth; neurotrophic factors; olfactory ensheathing cells; peripheral nerve; regeneration; SPINAL-CORD; NEUROTROPHIC FACTOR; GROWTH-FACTOR; IN-VITRO; NEURON REGENERATION; SENSORY NEURONS; STEM-CELLS; GLIA; REMYELINATION; NEUROGENESIS;

D O I：

10.1002/mus.21907

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Introduction: Olfactory ensheathing cells (OECs) hold promise for cell therapy because they may promote regeneration of the central nervous system. However, OECs have been less studied after peripheral nerve injury (PNI). The purpose of this investigation was to determine the effect of OEC transplantation on a severe sciatic nerve (SN) lesion. Methods: OECs were injected in rats after section and 2-cm resection of the SN. Results: Three months after therapy, muscle strength and morphometric studies showed complete restoration of the contractile properties of the gastrocnemius and complete repair of the SN. Immunohistochemistry and RT-PCR studies indicated an increase in the presence of neurotrophic factors. Interestingly, tracking of green fluorescent protein (GFP)-positive OECs showed that no OECs were present in the SN. Discussion: Our results demonstrate that, after severe PNI, OECs have remarkable potential for nerve regeneration by creating a favorable microenvironment. Muscle Nerve 43: 543-551, 2011

引用

页码：543 / 551

页数：9

共 47 条

[1] Olfactory ensheathing cell transplantation restores functional deficits in rat model of Parkinson's disease: a cotransplantation approach with fetal ventral mesencephalic cells [J].