Differentiation of Mycoplasma species by 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis fingerprinting

被引：56

作者：

McAuliffe, L

Ellis, RJ

Ayling, RD

Nicholas, RAJ

机构：

[1] Vet Labs Agcy Weybridge, Dept Bacterial Dis, Mycoplasma Grp, Addlestone KT15 3NB, Surrey, England

[2] Univ London Imperial Coll Sci Technol & Med, NERC, Ctr Populat Biol, Ascot SL5 7PY, Berks, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 10期

关键词：

D O I：

10.1128/JCM.41.10.4844-4847.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species of veterinary significance. Twenty-seven (85%) species could be differentiated by DGGE. This method could enable the rapid identification of many mycoplasma species for which there is no specific PCR available and which are currently identified by using culture and serological tests.

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页码：4844 / 4847

页数：4

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