Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine

被引:43
|
作者
Ma, Mingfang [1 ]
Wen, Kai [2 ,3 ]
Beier, Ross C. [4 ]
Eremin, Sergei A. [5 ]
Li, Chenglong [1 ]
Zhang, Suxia [1 ,2 ,3 ]
Shen, Jianzhong [1 ,2 ,3 ]
Wang, Zhanhui [1 ,2 ,3 ]
机构
[1] China Agr Univ, Coll Vet Med, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China
[2] Beijing Lab Food Qual & Safety, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China
[3] Beijing Key Lab Detect Technol Anim Derived Food, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China
[4] USDA ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77845 USA
[5] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119992, Russia
基金
中国国家自然科学基金;
关键词
quantum dots; chemiluminescence resonance energy transfer; immunoassay; hapten functionalization; chemical contaminant; LINKED-IMMUNOSORBENT-ASSAY; SULFONAMIDES; WATER; ANTIBODIES; PLATFORM; SAMPLES; MILK;
D O I
10.1021/acsami.6b04171
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing hapten-functionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenyl-sulfonamido)butanoic acid. The CRET-based immunoassay exhibited a limit of detection for SMZ of 9 pg mL(-1), which is >4 orders of magnitude better than a homogeneous fluorescence polarization immunoassay and is 2 orders of magnitude better than a heterogeneous enzyme-linked immunosorbent assay. This strategy represents a simple, reliable, and universal approach for detection of chemical contaminants.
引用
收藏
页码:17745 / 17750
页数:6
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