Aptasensor for Oncoprotein Platelet-Derived Growth Factor Detection on Functionalized Diamond Surface by Signal-Off Optical Method

被引：9
作者：
Ishii, Yoko [1 ]
Tajima, Shinya [1 ]
Kawarada, Hiroshi [1 ]
机构：
[1] Waseda Univ, Dept Elect Engn & Biosci, Sch Sci & Engn, Shinjuku Ku, Tokyo 1698555, Japan
来源：
APPLIED PHYSICS EXPRESS | 2011年 / 4卷 / 02期
关键词：
LABEL-FREE; GOLD NANOPARTICLES; THIN-FILMS; IN-VIVO; APTAMER; DNA; BIOSENSOR; REAGENTLESS; LIGANDS; SWITCH;
D O I：
10.1143/APEX.4.027001
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM. (C) 2011 The Japan Society of Applied Physics
引用
收藏
页数：3
相关论文
共 43 条
  • [31] Aptamer-Functionalized Hybrid Carbon Nanofiber FET-Type Electrode for a Highly Sensitive and Selective Platelet-Derived Growth Factor Biosensor
    Jun, Jaemoon
    Lee, Jun Seop
    Shin, Dong Hoon
    Jang, Jyongsik
    ACS APPLIED MATERIALS & INTERFACES, 2014, 6 (16) : 13859 - 13865
  • [32] A sensitive biosensor based on a ferrocene-marked adapter for the fluorescence detection of platelet-derived growth factor BB
    Lu, Xia
    Wen, Xiaoye
    Fan, Zhefeng
    JOURNAL OF LUMINESCENCE, 2020, 221 (221)
  • [33] Carbon-based nanocomposites with aptamer-templated silver nanoclusters for the highly sensitive and selective detection of platelet-derived growth factor
    Zhang, Zhihong
    Guo, Chuanpan
    Zhang, Shuai
    He, Linghao
    Wang, Minghua
    Peng, Donglai
    Tian, Junfeng
    Fang, Shaoming
    BIOSENSORS & BIOELECTRONICS, 2017, 89 : 735 - 742
  • [34] A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex
    Lu, Lihua
    Mao, Zhifeng
    Kang, Tian-Shu
    Leung, Chung-Hang
    Ma, Dik-Lung
    BIOSENSORS & BIOELECTRONICS, 2016, 85 : 300 - 309
  • [35] Dual Aptamer-Functionalized in Situ Injectable Fibrin Hydrogel for Promotion of Angiogenesis via Codelivery of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor-BB
    Zhao, Nan
    Suzuki, Akiho
    Zhang, Xiaolong
    Shi, Peng
    Abune, Lidya
    Coyne, James
    Jia, Huizhen
    Xiong, Na
    Zhang, Ge
    Wang, Yong
    ACS APPLIED MATERIALS & INTERFACES, 2019, 11 (20) : 18123 - 18132
  • [36] Ultrasensitive sensing platform for platelet-derived growth factor BB detection based on layered molybdenum selenide-graphene composites and Exonuclease III assisted signal amplification
    Huang, Ke-Jing
    Shuai, Hong-Lei
    Zhang, Ji-Zong
    BIOSENSORS & BIOELECTRONICS, 2016, 77 : 69 - 75
  • [37] 12/15-Lipoxygenase Contributes to Platelet-derived Growth Factor-induced Activation of Signal Transducer and Activator of Transcription 3
    Blazevic, Tina
    Schwaiberger, Andrea V.
    Schreiner, Cornelia E.
    Schachner, Daniel
    Schaible, Anja M.
    Grojer, Christoph S.
    Atanasov, Atanas G.
    Werz, Oliver
    Dirsch, Verena M.
    Heiss, Elke H.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (49) : 35592 - 35603
  • [38] Label-Free Electrochemical Detection of the Cancer Biomarker Platelet-Derived Growth Factor Receptor in Human Serum and Cancer Cells
    Rejeeth, Chandrababu
    Sharma, Alok
    Kannan, Soundarapandian
    Kumar, Raju Suresh
    Almansour, Abdulrahman, I
    Arumugam, Natarajan
    Afewerki, Samson
    ACS BIOMATERIALS SCIENCE & ENGINEERING, 2022, 8 (02) : 826 - 833
  • [39] Protein-templated cobaltous phosphate nanocomposites for the highly sensitive and selective detection of platelet-derived growth factor-BB
    He, Linghao
    Zhang, Shuai
    Ji, Hongfei
    Wang, Minghua
    Peng, Donglai
    Yan, Fufeng
    Fang, Shaoming
    Zhang, Hongzhong
    Jia, Chunxiao
    Zhang, Zhihong
    BIOSENSORS & BIOELECTRONICS, 2016, 79 : 553 - 560
  • [40] Immobilized rolling circle amplification on extended-gate field-effect transistors with integrated readout circuits for early detection of platelet-derived growth factor
    Lin, Ming-Yu
    Hsu, Wen-Yang
    Yang, Yuh-Shyong
    Huang, Jo-Wen
    Chung, Yueh-Lin
    Chen, Hsin
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2016, 408 (17) : 4785 - 4797
12345