Aptasensor for Oncoprotein Platelet-Derived Growth Factor Detection on Functionalized Diamond Surface by Signal-Off Optical Method

被引：9
|
作者：
Ishii, Yoko [1 ]
Tajima, Shinya [1 ]
Kawarada, Hiroshi [1 ]
机构：
[1] Waseda Univ, Dept Elect Engn & Biosci, Sch Sci & Engn, Shinjuku Ku, Tokyo 1698555, Japan
来源：
APPLIED PHYSICS EXPRESS | 2011年 / 4卷 / 02期
关键词：
LABEL-FREE; GOLD NANOPARTICLES; THIN-FILMS; IN-VIVO; APTAMER; DNA; BIOSENSOR; REAGENTLESS; LIGANDS; SWITCH;
D O I：
10.1143/APEX.4.027001
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
The highly sensitive detection of platelet-derived growth factor (PDGF) has been realized using aptamers immobilized on a diamond surface. The detection mechanism of PDGF is based on the release of an intercalating dye from the aptamer's stem structure during deformation when the aptamer captures PDGF. The detection by fluorescence reduction (signal-off method) does not require a target label and is unaffected by nonspecific binding. The high reproducibility of detection was confirmed. Since aptamers bind to their target PDGF with high affinity, the sensor exhibited high selectivity and sensitivity. The quantitation limit was determined to be 1 pM. (C) 2011 The Japan Society of Applied Physics
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