Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles

被引：405

作者：

Fan, Jia ^{[1
,2
]}

Yin, Jun-Jie ^{[3
]}

Ning, Bo ^{[2
]}

Wu, Xiaochun ^{[2
]}

Hu, Ye ^{[4
]}

Ferrari, Mauro ^{[4
]}

Anderson, Gregory J. ^{[5
]}

Wei, Jingyan ^{[1
]}

Zhao, Yuliang ^{[6
]}

Nie, Guangjun ^{[2
]}

机构：

[1] Jilin Univ, Coll Pharmaceut Sci, Changchun 130021, Peoples R China

[2] Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[3] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA

[4] Univ Texas Hlth Sci Ctr Houston, Dept Nanomed & Biomed Engn, Houston, TX 77031 USA

[5] Royal Brisbane Hosp, Queensland Inst Med Res, Brisbane, Qld 4029, Australia

[6] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100864, Peoples R China

来源：

BIOMATERIALS | 2011年 / 32卷 / 06期

关键词：

Biomimetic enzymes; Catalase activities; ESR; Ferritin; Nanoparticles; OXIDE NANOPARTICLES; METHANOL OXIDATION; APOFERRITIN; PROTEIN; SUPEROXIDE; TOXICOLOGY; STRESS; CARBON; CERIUM; CAGE;

D O I：

10.1016/j.biomaterials.2010.11.004

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Using apoferritin (apoFt) as a nucleation substrate, we have successfully synthesized 1-2 nm platinum nanoparticles (Pt-Ft) which are highly stable. By directly measuring the products of Pt-Ft-catalyzed reactions, we showed, with no doubt, Pt-Ft possesses both catalase and peroxidase activities. With hydrogen peroxide as substrate, we observed oxygen gas bubbles were generated from hydrogen peroxide decomposed by Pt-Ft: the generation of oxygen gas strongly supports Pt-Ft reacts as catalase, other than peroxidase. While with organic dyes and hydrogen peroxide as substrates, distinctive color products were formed catalyzed by Pt-Ft, which indicates a peroxidase-like activity. Interestingly, these biomimetic properties showed differential response to pH and temperature for different reaction substrates. Pt-Ft showed a significant increase in catalase activity with increasing pH and temperature. The HRP-like activity of Pt-Ft was optimal at physiological temperature and slightly acidic conditions. Our current study demonstrates that Pt-Ft possesses both catalase and peroxidase activities for different substrates under different conditions. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1611 / 1618

页数：8

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