Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles

被引：385

作者：

Fan, Jia ^{[1
,2
]}

Yin, Jun-Jie ^{[3
]}

Ning, Bo ^{[2
]}

Wu, Xiaochun ^{[2
]}

Hu, Ye ^{[4
]}

Ferrari, Mauro ^{[4
]}

Anderson, Gregory J. ^{[5
]}

Wei, Jingyan ^{[1
]}

Zhao, Yuliang ^{[6
]}

Nie, Guangjun ^{[2
]}

机构：

[1] Jilin Univ, Coll Pharmaceut Sci, Changchun 130021, Peoples R China

[2] Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[3] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA

[4] Univ Texas Hlth Sci Ctr Houston, Dept Nanomed & Biomed Engn, Houston, TX 77031 USA

[5] Royal Brisbane Hosp, Queensland Inst Med Res, Brisbane, Qld 4029, Australia

[6] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100864, Peoples R China

来源：

BIOMATERIALS | 2011年 / 32卷 / 06期

关键词：

Biomimetic enzymes; Catalase activities; ESR; Ferritin; Nanoparticles; OXIDE NANOPARTICLES; METHANOL OXIDATION; APOFERRITIN; PROTEIN; SUPEROXIDE; TOXICOLOGY; STRESS; CARBON; CERIUM; CAGE;

D O I：

10.1016/j.biomaterials.2010.11.004

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Using apoferritin (apoFt) as a nucleation substrate, we have successfully synthesized 1-2 nm platinum nanoparticles (Pt-Ft) which are highly stable. By directly measuring the products of Pt-Ft-catalyzed reactions, we showed, with no doubt, Pt-Ft possesses both catalase and peroxidase activities. With hydrogen peroxide as substrate, we observed oxygen gas bubbles were generated from hydrogen peroxide decomposed by Pt-Ft: the generation of oxygen gas strongly supports Pt-Ft reacts as catalase, other than peroxidase. While with organic dyes and hydrogen peroxide as substrates, distinctive color products were formed catalyzed by Pt-Ft, which indicates a peroxidase-like activity. Interestingly, these biomimetic properties showed differential response to pH and temperature for different reaction substrates. Pt-Ft showed a significant increase in catalase activity with increasing pH and temperature. The HRP-like activity of Pt-Ft was optimal at physiological temperature and slightly acidic conditions. Our current study demonstrates that Pt-Ft possesses both catalase and peroxidase activities for different substrates under different conditions. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1611 / 1618

页数：8

共 39 条

[1] Aime S, 2002, ANGEW CHEM INT EDIT, V41, P1017, DOI 10.1002/1521-3773(20020315)41:6<1017::AID-ANIE1017>3.0.CO
[2] 2-P
[3] Oxidase-Like Activity of Polymer-Coated Cerium Oxide Nanoparticles
Asati, Atul
Santra, Santimukul
Kaittanis, Charalambos
Nath, Sudip
Perez, J. Manuel
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (13) : 2308 - 2312
[4] Reduction of hexavalent chromium by human cytochrome b5:: Generation of hydroxyl radical and superoxide
Borthiry, Griselda R.
Antholine, William E.
Kalyanaraman, B.
Myers, Judith M.
Myers, Charles R.
[J]. FREE RADICAL BIOLOGY AND MEDICINE, 2007, 42 (06) : 738 - 755
[5] Rare earth nanoparticles prevent retinal degeneration induced by intracellular peroxides
Chen, Junping
Patil, Swanand
Seal, Sudipta
McGinnis, James F.
[J]. NATURE NANOTECHNOLOGY, 2006, 1 (02) : 142 - 150
[6] Nanostructured FeS as a Mimic Peroxidase for Biocatalysis and Biosensing
Dai, Zhihui
Liu, Shaohua
Bao, Jianchun
Jui, Huangxian
[J]. CHEMISTRY-A EUROPEAN JOURNAL, 2009, 15 (17) : 4321 - 4326
[7] Catalase and glutathione peroxidase mimics
Day, Brian J.
[J]. BIOCHEMICAL PHARMACOLOGY, 2009, 77 (03) : 285 - 296
[8] Biological synthesis of platinum nanoparticles with apoferritin
Deng, Q. Y.
Yang, B.
Wang, J. F.
Whiteley, C. G.
Wang, X. N.
[J]. BIOTECHNOLOGY LETTERS, 2009, 31 (10) : 1505 - 1509
[9] Protein- and Peptide-Directed Syntheses of Inorganic Materials
Dickerson, Matthew B.
Sandhage, Kenneth H.
Naik, Rajesh R.
[J]. CHEMICAL REVIEWS, 2008, 108 (11) : 4935 - 4978
[10] Intrinsic peroxidase-like activity of ferromagnetic nanoparticles
Gao, Lizeng
Zhuang, Jie
Nie, Leng
Zhang, Jinbin
Zhang, Yu
Gu, Ning
Wang, Taihong
Feng, Jing
Yang, Dongling
Perrett, Sarah
Yan, Xiyun
[J]. NATURE NANOTECHNOLOGY, 2007, 2 (09) : 577 - 583

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