Risk Factors for Antimicrobial Resistance in Turkey Farms: A Cross-Sectional Study in Three European Countries

被引：10

作者：

Horie, Mayu ^{[1
]}

Yang, Dongsheng ^{[1
]}

Joosten, Philip ^{[2
]}

Munk, Patrick ^{[3
]}

Wadepohl, Katharina ^{[4
]}

Chauvin, Claire ^{[5
]}

Moyano, Gabriel ^{[6
,7
]}

Skarzynska, Magdalena ^{[8
]}

Dewulf, Jeroen ^{[2
]}

Aarestrup, Frank M. ^{[3
]}

Blaha, Thomas ^{[4
]}

Sanders, Pascal ^{[5
]}

Gonzalez-Zorn, Bruno

Wasyl, Dariusz ^{[8
]}

Wagenaar, Jaap A. ^{[9
,10
]}

Heederik, Dick ^{[1
]}

Mevius, Dik ^{[9
,10
]}

Schmitt, Heike ^{[1
,11
]}

Smit, Lidwien A. M. ^{[1
]}

Van Gompel, Liese ^{[1
]}

机构：

[1] Univ Utrecht, Inst Risk Assessment Sci, Yalelaan 2, NL-3584 CM Utrecht, Netherlands

[2] Univ Ghent, Dept Obstet Reprod & Herd Hlth, Vet Epidemiol Unit, Salisburylaan 133, B-9820 Merelbeke, Belgium

[3] Tech Univ Denmark, Natl Food Inst, Res Grp Genom Epidemiol, DK-2800 Lyngby, Denmark

[4] Univ Vet Med Hannover, Field Stn Epidemiol, Buscheler Str 9, D-49456 Bakum, Germany

[5] French Agcy Food Environm & Occupat Hlth & Safety, Epidemiol Hlth & Welf Unit, F-22440 Ploufragan, France

[6] Univ Complutense Madrid, Fac Vet Med, Dept Anim Hlth, Antimicrobial Resistance Unit ARU, Madrid 28040, Spain

[7] Univ Complutense Madrid, VISAVET Hlth Surveillance Ctr, Madrid 28040, Spain

[8] Natl Vet Res Inst PIWet, Dept Microbiol, Partyzantow Ave 57, PL-24100 Pulawy, Poland

[9] Univ Utrecht, Dept Infect Dis & Immunol, Fac Vet Med, Yalelaan 1, NL-3584 CL Utrecht, Netherlands

[10] Wageningen Biovet Res, Dept Bacteriol & Epidemiol, Houtribweg 39, NL-8221 RA Lelystad, Netherlands

[11] Natl Inst Publ Hlth & Environm, POB 1, NL-3720 BA Bilthoven, Netherlands

来源：

ANTIBIOTICS-BASEL | 2021年 / 10卷 / 07期

基金：

欧盟第七框架计划;

关键词：

antimicrobial use; antimicrobial resistance; turkeys; poultry; farm; antimicrobial resistance genes; biosecurity; risk factor; metagenomics; qPCR; isolates; ESCHERICHIA-COLI; GENES; ASSOCIATION; ABUNDANCE; CARRIAGE; PACKAGE; SPP; TETW; ERMB;

D O I：

10.3390/antibiotics10070820

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Food-producing animals are an important reservoir and potential source of transmission of antimicrobial resistance (AMR) to humans. However, research on AMR in turkey farms is limited. This study aimed to identify risk factors for AMR in turkey farms in three European countries (Germany, France, and Spain). Between 2014 and 2016, faecal samples, antimicrobial usage (AMU), and biosecurity information were collected from 60 farms. The level of AMR in faecal samples was quantified in three ways: By measuring the abundance of AMR genes through (i) shotgun metagenomics sequencing (n = 60), (ii) quantitative real-time polymerase chain reaction (qPCR) targeting ermB, tetW, sul2, and aph3 '-III; (n = 304), and (iii) by identifying the phenotypic prevalence of AMR in Escherichia coli isolates by minimum inhibitory concentrations (MIC) (n = 600). The association between AMU or biosecurity and AMR was explored. Significant positive associations were detected between AMU and both genotypic and phenotypic AMR for specific antimicrobial classes. Beta-lactam and colistin resistance (metagenomics sequencing); ampicillin and ciprofloxacin resistance (MIC) were associated with AMU. However, no robust AMU-AMR association was detected by analyzing qPCR targets. In addition, no evidence was found that lower biosecurity increases AMR abundance. Using multiple complementary AMR detection methods added insights into AMU-AMR associations at turkey farms.

引用

页数：20