Proton translocating ATPase mediated fungicidal activity of eugenol and thymol

被引：107

作者：

Ahmad, Aijaz ^{[1
]}

Khan, Amber ^{[1
]}

Yousuf, Snowber ^{[1
]}

Khan, Luqman A. ^{[1
]}

Manzoor, Nikhat ^{[1
]}

机构：

[1] Jamia Millia Islamia, Dept Biosci, New Delhi 110025, India

来源：

FITOTERAPIA | 2010年 / 81卷 / 08期

关键词：

Eugenol; Thymol; Plasma membrane H+-ATPase; Intracellular pH; Candida; CANDIDA-ALBICANS; LISTERIA-MONOCYTOGENES; ANTIMICROBIAL ACTIVITY; ESCHERICHIA-COLI; ESSENTIAL OILS; INHIBITION; RESISTANCE; SYNTHASE; SACCHAROMYCES; POLYPHENOLS;

D O I：

10.1016/j.fitote.2010.07.020

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Eugenol (1) and thymol (2) exhibit excellent fungicidal activity against pathogenic yeasts, including isolates resistant to azoles. The rapid irreversible action of compound-1 and compound 2 on fungal cells suggested a membrane-located target for their action. We investigated their effect on H+-ATPase mediated H+-pumping by various Candida species. Both compounds inhibit H+-ATPase activity at their respective MIC values - 500 and 100 mu g/ml. Glucose stimulated H+-extrusion was also inhibited significantly by compound 1 and compound 2. Inhibition of H+-ATPase leads to intracellular acidification and cell death. Inhibition of cell growth and H+-efflux by test compounds suggests that their antifungal properties are related to their inhibitory effects on H+-ATPase. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1157 / 1162

页数：6

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