Polarization-resolved second-harmonic-generation imaging of dermal collagen fiber in pre-wrinkled skin of ultraviolet-B-exposed mouse

被引：0

作者：

Fukushima, Shu-ichiro ^{[1
]}

Yonetsu, Makoto ^{[1
]}

Hase, Eiji ^{[2
,3
]}

Yasui, Takeshi ^{[1
,2
]}

机构：

[1] Osaka Univ, Grad Sch Engn Sci, 1-3 Machikaneyama, Toyonaka, Osaka 5608531, Japan

[2] Tokushima Univ, Grad Sch Technol Ind & Social Sci, 2-1 Minami Josanjima, Tokushima, Tokushima 7708506, Japan

[3] JASRI SPring 8, 1-1-1 Sayo, Sayo, Hyogo 6795198, Japan

来源：

BIOMEDICAL IMAGING AND SENSING CONFERENCE | 2018年 / 10711卷

关键词：

skin; photoaging; collagen; wrinkle; orientation; second-harmonic-generation microscopy; HUMAN TISSUE; ORIENTATION; POLARIMETRY; MICROSCOPY; LIGHT;

D O I：

10.1117/12.2319360

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Wrinkling is a typical symptom of cutaneous photoaging in skin; however, mechanism of such wrinkling is still not clear. In this paper, we investigated orientation change of dermal collagen fiber in pre-wrinkled skin of ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved second-harmonic-generation (SHG) microscopy. A polarization anisotropic image of the SHG light indicated that change of collagen fiber orientation started in the pre-wrinkled skin of ultraviolet-B-exposed (UVB-exposed) mouse. Furthermore, the dominant direction of collagen fiber orientation was significantly parallel to the wrinkle direction in post-wrinkled skin of UVB-exposed mouse. This result implies that change of collagen fiber orientation is a trigger of wrinkling in photo-aged skin.

引用

页数：4

共 7 条

[1] Second harmonic generation microscopy: principles and applications to disease diagnosis [J].

Campagnola, Paul J. ;

Dong, Chen-Yuan .

LASER & PHOTONICS REVIEWS, 2011, 5 (01) :13-26

[2] SKIN AGING AND PHOTOAGING - AN OVERVIEW [J].

GILCHREST, BA .

JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1989, 21 (03) :610-613

[3] THE ULTRAVIOLET-IRRADIATED HAIRLESS MOUSE - A MODEL FOR PHOTOAGING [J].

KLIGMAN, LH .

JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1989, 21 (03) :623-631

[4] Tomographic imaging of collagen fiber orientation in human tissue using depth-resolved polarimetry of second-harmonic-generation light [J].

Yasui, T ;

Sasaki, K ;

Tohno, Y ;

Araki, T .

OPTICAL AND QUANTUM ELECTRONICS, 2005, 37 (13-15) :1397-1408

[5] Determination of collagen fiber orientation in human tissue by use of polarization measurement of molecular second-harmonic-generation light [J].

Yasui, T ;

Tohno, Y ;

Araki, T .

APPLIED OPTICS, 2004, 43 (14) :2861-2867

[6] Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry [J].

Yasui, T ;

Tohno, Y ;

Araki, T .

JOURNAL OF BIOMEDICAL OPTICS, 2004, 9 (02) :259-264

[7] Observation of dermal collagen fiber in wrinkled skin using polarization-resolved second-harmonic-generation microscopy [J].

Yasui, Takeshi ;

Takahashi, Yu ;

Fukushima, Shuichiro ;

Ogura, Yuki ;

Yamashita, Toyonobu ;

Kuwahara, Tomohiro ;

Hirao, Tetsuji ;

Araki, Tsutomu .

OPTICS EXPRESS, 2009, 17 (02) :912-923

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